New insights on CRISPR/Cas9-based therapy for breast Cancer

Genes and Environment

Table 4 A group of web-based algorithm for gRNA designing

Tool name	Off-target analysis	Webpage link
CRISPR Design Tool	Yes	https://www.synthego.com/products/bioinformatics/crispr-design-tool/
Cas-Designer	No	http://www.rgenome.net/cas-designer/
CRISPR MultiTargeter	No	http://www.multicrispr.net/
CRISPR Genome Analysis Tool		http://cbc.gdcb.iastate.edu/cgat/
GeneArt	Yes	https://www.thermofisher.com/sa/en/home/life-science/genome-editing/geneart-crispr/geneart-crispr-search-and-design-tool.html/
ZiFiT Targeter Version 4.2	No	http://zifit.partners.org/ZiFiT/ChoiceMenu.aspx/
CRISPR/Cas9 target online predictor	Yes	https://crispr.cos.uni-heidelberg.de/
Cas9 online designer	Yes	http://www.rgenome.net/cas-designer/
CHOPCHOP	Yes	http://chopchop.cbu.uib.no/
sgRNAcas9	Yes	http://www.biootools.com/col.jsp?id=103/
E-CRISP	Yes	http://www.e-crisp.org/E-CRISP/

ISSN: 1880-7062