Table 1 The gpt mutation spectra in the liver of BaP and ENU-treated homozygous gpt delta rats
0 mg/kg/day (28 + 3d) | BaP 125 mg/kg/day (28 + 3d) | ENU 50 mg/kg/day (5 + 26d) | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
male | female | male | female | male | female | |||||||||||||
No. | % | MF (x10− 6) | No. | % | MF (x10− 6) | No. | % | MF (x10− 6) | No. | % | MF (x10− 6) | No. | % | MF (x10− 6) | No. | % | MF (x10− 6) | |
Base substitution | ||||||||||||||||||
Transition | ||||||||||||||||||
G:C to A:T | 15 | 40.5 | 2.2 | 17 | 45.9 | 3.0 | 5 | 12.2 | 6.8 | 7 | 16.3 | 16.2 | 7 | 15.6 | 14.9 | 9 | 21.4 | 18.1 |
(at CpG) | (6) | (10) | (3) | (1) | (1) | (2) | ||||||||||||
A:T to G:C | 4 | 10.8 | 0.6 | 2 | 5.4 | 0.3 | 2 | 4.9 | 2.7 | 0 | 0.0 | 0.0 | 10 | 22.2 | 21.3 | 5 | 11.9 | 10.1 |
Transversion | ||||||||||||||||||
G:C to T:A | 6 | 16.2 | 0.9 | 8 | 21.6 | 1.4 | 19 | 46.3 | 25.7 | 15 | 34.9 | 34.8 | 7 | 15.6 | 14.9 | 4 | 9.5 | 8.1 |
G:C to C:G | 0 | 0.0 | 0.0 | 3 | 8.1 | 0.5 | 6 | 14.6 | 8.1 | 9 | 20.9 | 20.9 | 0 | 0.0 | 0.0 | 1 | 2.4 | 2.0 |
A:T to T:A | 0 | 0.0 | 0.0 | 1 | 2.7 | 0.2 | 0 | 0.0 | 0.0 | 2 | 4.7 | 4.6 | 11 | 24.4 | 23.4 | 14 | 33.3 | 28.2 |
A:T to C:G | 1 | 2.7 | 0.1 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 | 2 | 4.7 | 4.6 | 7 | 15.6 | 14.9 | 7 | 16.7 | 14.1 |
Deletion | 8 | 21.6 | 1.2 | 5 | 13.5 | 0.9 | 6 | 14.6 | 8.1 | 5 | 11.6 | 11.6 | 3 | 6.7 | 6.4 | 1 | 2.4 | 2.0 |
1 bp | 6 | 4 | 3 | 3 | 2 | 1 | ||||||||||||
> 2 bps | 2 | 1 | 3 | 2 | 1 | 0 | ||||||||||||
Insertion | 2 | 5.4 | 0.3 | 0 | 0.0 | 0.0 | 2 | 4.9 | 2.7 | 1 | 2.3 | 2.3 | 0 | 0.0 | 0.0 | 1 | 2.4 | 2.0 |
Others | 1 | 2.7 | 0.1 | 1 | 2.7 | 0.2 | 1 | 2.4 | 1.4 | 2 | 4.7 | 4.6 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Total | 37 | 100 | 5.3 | 37 | 100 | 6.5 | 41 | 100 | 55.4 | 43 | 100 | 99.7 | 45 | 100 | 95.9 | 42 | 100 | 84.7 |