Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides

Genes and Environment

Table 1 DNA Adducts Formed in Reaction with 2-Chlorooxirane (Vinyl Chloride Epoxide)

Adduct^a	Yield with 10 mM 2-chlorooxirane, pmol (μmol of DNA base)^− 1	Method	Ref
N⁷-(2-oxoethyl)Gua	10,000	HPLC/fluorescence	[34]
1,N⁶-ε-dAdo	500	HPLC/fluorescence	[34]
HO-ethanoGua	24	HPLC/MS/MS	[35]
HO-ethanodGuo	29	HPLC/MS/MS	[35]
N²,3-ε-Gua	16	HPLC/fluorescence	[1]
3,N⁴-ε-dCyd	7	HPLC/fluorescence	[35]
1,N²-ε-Gua	~1^b	HPLC/fluorescence	[1]
	2.5	HPLC/MS/MS	[35]

^aThe name of the adduct indicates whether the base or deoxyribonucleoside was assayed
^bIn other work, a 15 mg mL^− 1 concentration of herring sperm DNA was used instead of 5 mg of calf thymus DNA mL^− 1 in references [34], and a value of 6.5 pmol (μmol of DNA base)^− 1 was obtained [1]

ISSN: 1880-7062