Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides

Genes and Environment

Table 2 Levels of Measured Endogenous Etheno DNA Adducts (see also [61])

DNA Source		Adducts/10⁸ nucleotides				Method	Reference
DNA Source		1,N⁶-ε-dAdo	3,N⁴-ε-dCyd	1,N²-ε-dGuo	N²,3-ε-dGuo	Method	Reference
Rat	Liver	0.10–0.12	0.10–0.12			Immunoaffinity/³²P labeling	[47]
	Liver				18–23	GC/MS	[47]
	Liver	120	560			HPLC/³²P labeling	[62]
	Liver	0.011–0.012	0.016–0.017			Immunoaffinity/³²P labeling	[63]
	Lung	0.4–0.5	1.8–3.8			Immunoaffinity/³²P labeling	[63]
	Kidney	0.7–0.8	2.1–2.8			Immunoaffinity/³²P labeling	[63]
	Lymphocytes	1.1	1.2			Immunoaffinity/³²P labeling	[63]
	Hepatocytes				1.4	Immunoaffinity/GC/MS	[64]
	Hepatocytes			ND^a		Immunoaffinity/GC/MS	[65]
Human	Hepatocytes				2.0	LC/MS	[66]
	Oral cells	0.77	0.61			LC/MS	[67]
	Placenta	74–84				LC/MS	[68]
	Placenta	1.1				LC/MS	[69]
	Pancreas	1.8	1.2			Immunoaffinity/³²P labeling	[70]

ISSN: 1880-7062