Genes and Environment

Table 3 N²,3-ε-dGuo vs. 1, N²-ε-dGuo [90, 105,106,107,108]

From: Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides


Polymerase	Template	dNTP	k_cat/K_m (μM^− 1 min^− 1)	f	Template	dNTP	k_cat/K_m (μM^− 1 min^− 1)	f
E. coli pol I KF (exo¯)	3′-G*TA-	N²,3-ε-dG:C	0.23	1		1,N²-ε-dG:C	0.0081	1
		N²,3-ε-dG:T	0.24	1	3′-G*TG-	1,N²-ε-dG:G	0.0087	1.1
						1,N²-ε-dG:A	0.0016	0.2
S. solfotaricus Dpo4	3′-G*TA-	N²,3-ε-dG:C	0.025	1	3′-G*TA-	1,N²-ε-dG:C	0.00006	1
S. solfotaricus Dpo4	3′-G*TA-	N²,3-ε-dG:T	0.0054	0.22	3′-G*TA-	1,N²-ε-dG:A	0.0008	14
Human pol κ	3′-G*TA-	N²,3-ε-dG:C	0.022	1	3′-G*TA-	1,N²-ε-dG:C	0.0012	1
Human pol κ	3′-G*TA-	N²,3-ε-dG:T	0.0081	0.37	3′-G*TA-	1,N²-ε-dG:T	0.0012	1
Human pol ι	3′-G*TA-	N²,3-ε-dG:C	0.0017	1	3′-G*TA-	1,N²-ε-dG:C	0.0017	1
Human pol ι	3′-G*TA-	N²,3-ε-dG:T	0.0012	0.71	3′-G*TA-	1,N²-ε-dG:T	0.016	9.6

G*: N²,3-ε-dG or 1,N²-ε-dG; f = (k_cat/K_m)_incorrect/(k_cat/K_m)_correct

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