Fig. 2From: Mutagenicity of carcinogenic heterocyclic amines in Salmonella typhimurium YG strains and transgenic rodents including gpt deltaProtocols of gpt delta transgenic rodent mutation assays. Gpt delta mice or rats are exposed to chemicals by feeding, gavage or others. Then, the genomic DNA is extracted from various organs or tissues to recover λ phage EG10 particles by λ packaging reactions. Then, the rescued phages are introduced to indicator E. coli for gpt selection and for Spi− selection that detect point mutations and deletion mutations, respectively. DNA is extracted from 6-TG-resistant colonies or Spi− plaques for DNA sequencingBack to article page