Fig. 1
Characterization of neoplastic-like transformed MCN-MeT-5A cells. MeT-5A cells were chronic exposed to MWCNT at 10 μg/cm2 for one year as described in the Materials and Methods to generate the MCN-MeT-5A cells. A MCN-MeT-5A cells exhibited increased proliferation compared to the parental MeT-5A cells. Cell number was counted by hemocytometer. The number counting was repeated at least three times, and error bar stands for standard deviation (SD). *P < 0.05 versus control cells. B Cell mounds was formed in MCN-MeT-5A cells. The cell morphology images were recorded using a Leica microscope. Bar = 500 μm. C Soft agar colony formation assay showed multiple colonies formed by MCN-MeT-5A cells but not the passage-matched control MeT-5A cells. Bar = 500 μm. D MCN-MeT-5A cells migrated faster than passage-matched control MeT-5A cells in the wound healing assay at both 24 h and 48 h after scratching. Bar = 500 μm