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Table 1 PCR primers and restricted endonucleases for MTHFR gene in genotyping process

From: Folate metabolism modifies chromosomal damage induced by 1,3-butadiene: results from a match-up study in China and in vitro experiments

SNP Primers PCR method Restricted endonucleases
Forward Reverse   
MTHFR 677 5′-TGAAGGAGAAGGTGTCTGCGGGA-3′ 5′-AGGACGGTGCGGTGAGAGTG-3’ RFLP Hinf1
MTHFR 1298 5′-CTTTGGGAGCTGAAGGACTAC-3’ 5′-CACTTTGTGACCATTCCGGTTTG-3’ RFLP Mbo2