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Fig. 3 | Genes and Environment

Fig. 3

From: Genetic interaction between RLM1 and F-box motif encoding gene SAF1 contributes to stress response in Saccharomyces cerevisiae

Fig. 3

Genetic interaction between SAF1 and RLM1 impacts chitin distribution and cellular growth response to cell wall stressors. A Representative Fluorescence images of Calcofluor white, specific to chitin in the cell wall, stained cells of WT, saf1∆, rlm1∆, saf1∆rlm1∆ strains. The cells of rlm1∆ and saf1∆rlm1∆ strains showed the altered chitin distribution in the cell wall when compared to cells of WT and saf1∆ strain. B Relative fluorescence intensity of the Calcofluor white stained cells of WT and mutant strains. The saf1∆rlm1∆ strain showed 10-fold increases in the intensity of fluorescence signal in comparison to WT cells. C Images of semi- quantitative spot assay for comparative analysis of cellular growth response of cells in presence of 30 μg/ml Calcofluor white and 0.0075% SDS of WT, saf1∆, rlm1∆, and saf1∆rlm1∆ strains. The cells lacking SAF1 and RLM1 together show tolerance to CFW compared to WT, however slight sensitivity to SDS in comparison to WT. The cells lacking RLM1 show sensitivity to SDS compared to WT cells

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