Table 3 List of primers sequences used for amplification of deletion cassette
Gene Name | Primer code | Sequence |
|---|---|---|
SAF1 | SAF1F | 5’CCAAAGGATATACTCTCAATTATAAATGA |
AAGCACATCCGGATCCCCGGGTTAATTAA-3’ | ||
SAF1R | 5’ACGGAATCCAAAATGCAAAATCGAAATGA | |
CACCTAAAAA TGAATTCGAGCTCGTTTAAAC-3’ | ||
RLM1 | RLM1F | 5’TAAATATTAAAGTGTCGCAAAACTATACTA |
TAGATACAACCGGATCCCCGGGTTAATTAA-3’ | ||
RLM1R | 5’TCTTATGCTTGGAATATTCATACTGGTCAA | |
ATTTTTTGGTGAATTCGAGCTCGTTTAAAC-3’ | ||
RLM1 | RLM1FL | 5’TAAATATTAAAGTGTCGCAAAACTATACTA |
TAGATACAACCCAACTGTGGGAATACTCAG-3 | ||
RLM1RL | 5’TCTTATGCTTGGAATATTCATACTGGTCAA | |
ATTTTTTGGTTTGGCCCGAAATTCCCCTAC-3 |