Fig. 3

Distribution of Alu and LINE1 methylation level in PM-exposed NHBE (A) and NHEK (B). Methylation level was expressed as a percentage of 5-methylcytosine divided by the sum of methylated and unmethylated cytosines. The mean ± 95% confidence interval of three independent pyrosequencing is shown. The comparisons of mean methylation levels were evaluated using ANOVA. Bonferroni-adjusted p-value was the result of comparing between the two groups. Cells maintained in culture medium with vehicle were used as control group (CTL^a, 0.1% DMSO; CTL^b, 1% PBS)