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Fig. 2 | Genes and Environment

Fig. 2

From: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Fig. 2

DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase α (panel A), DNA polymerase β (panel B) and Kf exo− (panels C and D). DNA polymerase α (100 μU), DNA polymerase β (250 μU) and Kf exo− (75 μU) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls. The background darkness of panel C has been adjusted in panel D, and only the part of the gel around the 30-mer full length products is shown

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Genes and Environment

ISSN: 1880-7062

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