Fig. 2

RP-HPLC chromatogram of a reaction mixture of nucleosides with α-TP detected at 230 nm (upper figure) and the reaction scheme (lower figure). A solution of 100 μM each of dCyd, dGuo, dThd, dAdo, and 5 mM α-TP was irradiated with UV through a 300-nm longpass filter in 100 mM potassium phosphate buffer at pH 7.4 and 37°C for 10 min. The insets are the UV spectra of Products 1 and 2. Reaction of dThd irradiated by UV in the presence of α-TP