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Table 2 Effects of additives on reactions of nucleosides and Trolox with UV light (>300 nm)a

From: α-Tocopherol phosphate as a photosensitizer in the reaction of nucleosides with UV light: formation of 5,6-dihydrothymidine

 

dCyd

dGuo

dThd

dAdo

5S-DHdThd

5R-DHdThd

Additives

(μM)

(μM)

(μM)

(μM)

(μM)

(μM)

None

94.9 ± 0.2

98.4 ± 0.2

86.6 ± 0.8

96.3 ± 0.3

<1

<1

1% EtOH

99.2 ± 0.4

100.2 ± 0.5

86.9 ± 0.6

98.1 ± 0.5

<1

<1

10 mM NaN3

100.4 ± 0.5

100.7 ± 0.5

92.8 ± 0.6

101.0 ± 0.4

<1

<1

  1. aThe mixed nucleoside solution with 5 mM Trolox in 100 mM potassium phosphate buffer at pH 7.4 and 37°C with or without 1% EtOH and 10 mM NaN3 was irradiated with UV light for 10 min. All reaction mixtures were analyzed by RP-HPLC. Means ± standard deviation (S.D.) (n = 3) are presented.