Fig. 3

Representative photomicrographs of the corneal epitheliums in the rabbits. Saline as the negative control, 1% ethidium bromide (EtBr), 3% methyl methanesulfonate (MMS), or 1% 4-nitroquinoline 1-oxide (4-NQO) was administered once to the eyes of rabbits. The corneas were collected at 24 h, and corneal sections were stained with hematoxylin and eosin. No histopathological changes in the corneal epithelium were observed in the saline (a), 1% EtBr (b), and 3% MMS-treated eyes (c). Moderate degeneration/necrosis was noted in the corneal epithelium in the 1% 4-NQO-treated eyes (d). Scale bars: 20 μm