Time-course changes in DNA damage of corneal epithelial cells in rabbits following ocular instillation with genotoxic compounds

Table 4 Change in viability of human epithelial cells treated with genotoxic compounds

Incubation time after removing the compounds^a	Cell viability (%)
Incubation time after removing the compounds^a	0.5% EtBr	0.5% MMS	0.001% 4-NQO
Non-treated	100.0 ± 2.8	100.0 ± 2.6	100.0 ± 1.5
2 h	89.3 ± 6.1^b	115.1 ± 5.1	139.2 ± 2.0
4 h	74.7 ± 8.0^b	108.1 ± 0.3	114.2 ± 14.5
6 h	73.1 ± 4.6^b	108.2 ± 4.9	122.7 ± 24.2
24 h	39.1 ± 1.3^b	108.5 ± 11.3	118.3 ± 13.9

Data are presented as the mean ± SD (n = 3)
EtBr Ethidium bromide, MMS Methyl methanesulfonate, 4-NQO 4-nitroquinoline 1-oxide
^aTime of incubation with fresh culture medium after a 1 min exposure to each compound
^bSignificantly lower than the non-treated group at 5% probability level (Dunnett’s multiple comparison test, one-tailed)

ISSN: 1880-7062