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Fig. 1 | Genes and Environment

Fig. 1

From: Copper-mediated DNA damage caused by purpurin, a natural anthraquinone

Fig. 1

Autoradiogram of 32P-5′-end-labeled DNA fragments incubated with purpurin in the presence of Cu(II). Reaction mixtures contained the 32P-5′-end-labeled 147-bp fragment, 20 μM/base calf thymus DNA, the indicated concentrations of purpurin and 20 μM CuCl2 in 200 μl of 4 mM sodium phosphate buffer (pH 7.8) containing 5 μM DTPA. After incubation at 37 °C for 1 h under light shielding, the DNA fragments were treated with or without hot piperidine and electrophoresed on a polyacrylamide gel

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