Fig. 2

Hepatic PNPLA3 gene and SIRT1 protein expression and H3K9ac level of PNPLA3 promoter in C57BL/6 mice with NAFLD induced by high-sucrose diet. A Visualized oil red O staining using a research-grade inverted microscope at 200 × magnification. B Relative mRNA level of liver PNPLA3 measured by qPCR (N = 5 mice per group)..C The recruitment of H3K9ac onto the PNPLA3 promoter detected by ChIP-qPCR using an anti-H3k9Ac antibody. Rabbit IgG was used as a mock antibody for negative control. The recruitment is expressed as fold enrichment over IgG (N = 4 mice per group). D The protein level of liver SIRT1 detected by western blot. Quantitative analysis of gray value on bands was performed using Image-Pro Plus software. Quantitative data are presented as the means ± SD (N = 3 independent experiments). Ctrl: control mice fed a normal chow diet, HSD: mice fed a high sucrose diet. *P < 0.05, **P < 0.01