Fig. 4

Gene expression of PNPLA and H3K9ac level of PNPLA3 promoter were regulated by SIRT1 in HGHI-treated HepG2 cells. HepG2 cells pretreated with pcDNA3.1-null or pcDNA3.1-SIRT1 were incubated in media containing 25 mM glucose. A Visualized oil red O staining using a research-grade inverted microscope at 200 × magnification. B Relative mRNA of PNPLA3 measured by qPCR. C The protein level of SIRT1 detected by western blot. Quantitative analysis of gray value on bands was performed using Image-Pro Plus software. D The recruitment of H3K9ac onto the PNPLA3 promoter detected by ChIP-qPCR using an anti-H3k9Ac antibody. Rabbit IgG was used as a mock antibody for negative control. The recruitment is expressed as fold enrichment over IgG. Biological replicates (N = 3) were performed per group. Quantitative data are presented as the means ± SD (N = 3 independent experiments).*P < 0.05; **P < 0.01; ***P < 0.001