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Fig. 5 | Genes and Environment

Fig. 5

From: SIRT1 mediates nutritional regulation of SREBP-1c-driven hepatic PNPLA3 transcription via modulation of H3k9 acetylation

Fig. 5

SIRT1 regulates SREBP-1c-driven PNPLA3 gene expression in HepG2 cells. A Effect of SIRT1 on basal and SREBP-1c-driven PNPLA3 gene expression. HepG2 cells were transfected respectively or cotransfected with pcDNA3.1- SREBP-1c and SIRT1. PNPLA3 mRNA levels were measured by qPCR. B Effect of SIRT1 on SREBP-1c enrichment on the PNPLA3 promoter. HepG2 cells were transfected with pcDNA3.1-SREBP and pcDNA3.1-SIRT1 alone, or were cotransfected both together for 24 h. The endogenous binding of SREBP-1c and PNPLA3 promoter was detected by ChIP-qPCR, which was performed using an anti-SREBP-1c antibody. Rabbit IgG was used as a mock antibody for negative control. The recruitment is expressed as fold enrichment over IgG. Biological replicates (N = 3) were performed per group. Quantitative data are presented as the means ± SD (N = 3 independent experiments). ***P < 0.001

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