Table 6 Types of EGFR gene mutations identified in this study
| Â | Coal-producing areas | Specimen type | Gender | Smoking history | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total | Yes | No | Â | Tissue | Plasma and pleural effusion cells | Male | Female | Â | Yes | No | Â | |||||||||||
N | % | N | % | N | % | p | N | % | N | % | p | N | % | N | % | p | N | % | N | % | p | |
Sensitizing mutations | ||||||||||||||||||||||
 L858R | 135 | 33.09 | 63 | 27.16 | 72 | 40.91 | 0.003 | 119 | 32.96 | 16 | 34.04 | 0.882 | 45 | 30.00 | 90 | 34.88 | 0.31 | 14 | 21.88 | 121 | 35.17 | 0.038 |
 19-del | 87 | 21.32 | 41 | 17.67 | 46 | 26.14 | 0.039 | 77 | 21.33 | 10 | 21.28 | 0.993 | 30 | 20.00 | 57 | 22.09 | 0.62 | 13 | 20.31 | 74 | 21.51 | 0.830 |
 G719X | 29 | 7.11 | 23 | 9.91 | 6 | 3.41 | 0.011 | 25 | 6.93 | 4 | 8.51 | 0.923 | 9 | 6.00 | 20 | 7.75 | 0.51 | 6 | 9.38 | 23 | 6.69 | 0.614 |
 G719X + L861X | 10 | 2.45 | 4 | 1.72 | 6 | 3.41 | 0.443 | 10 | 2.77 | 0 | 0.00 | 0.513 | 3 | 2.00 | 7 | 2.71 | 0.91 | 1 | 1.56 | 9 | 2.62 | 0.952 |
 L861Q | 6 | 1.47 | 2 | 0.86 | 4 | 2.27 | 0.224 | 4 | 1.11 | 2 | 4.26 | 0.144 | 1 | 0.67 | 5 | 1.94 | 0.29 | 1 | 1.56 | 5 | 1.45 | 0.643 |
 L858R + L833F | 4 | 0.98 | 4 | 1.72 | 0 | 0.00 | 0.103 | 4 | 1.11 | 0 | 0.00 | 0.612 | 1 | 0.67 | 3 | 1.16 | 0.53 | 1 | 1.56 | 3 | 0.87 | 0.496 |
 G719X + E709X | 4 | 0.98 | 3 | 1.29 | 1 | 0.57 | 0.421 | 4 | 1.11 | 0 | 0.00 | 0.612 | 1 | 0.67 | 3 | 1.16 | 0.53 | 0 | 0.00 | 4 | 1.16 | 0.504 |
 L861X + L833F | 4 | 0.98 | 3 | 1.29 | 1 | 0.57 | 0.421 | 4 | 1.11 | 0 | 0.00 | 0.612 | 2 | 1.33 | 2 | 0.78 | 0.47 | 1 | 1.56 | 3 | 0.87 | 0.496 |
 L858R + EGFR gene ampilification | 3 | 0.74 | 2 | 0.86 | 1 | 0.57 | 0.603 | 3 | 0.83 | 0 | 0.00 | 0.692 | 1 | 0.67 | 2 | 0.78 | 0.69 | 0 | 0.00 | 3 | 0.87 | 0.599 |
 EGFR gene ampilification | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 1 | 1.56 | 0 | 0.00 | 0.157 |
 G719X + L858R | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 L833V + H835L | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 1 | 1.56 | 0 | 0.00 | 0.157 |
 18-del | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 19-del + EGFR gene ampilification | 3 | 0.74 | 0 | 0.00 | 3 | 1.70 | 0.079 | 3 | 0.83 | 0 | 0.00 | 0.692 | 1 | 0.67 | 2 | 0.78 | 0.69 | 1 | 1.56 | 2 | 0.58 | 0.401 |
 L858R + E709X | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 1 | 0.28 | 0 | 0.00 | 0.885 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 L861Q + L838V | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 1 | 1.56 | 0 | 0.00 | 0.157 |
Resistance mutations | ||||||||||||||||||||||
 20INS | 14 | 3.43 | 5 | 2.16 | 9 | 5.11 | 0.104 | 13 | 3.60 | 1 | 2.13 | 0.923 | 6 | 4.00 | 8 | 3.10 | 0.63 | 3 | 4.69 | 11 | 3.20 | 0.820 |
 S768I | 5 | 1.23 | 5 | 2.16 | 0 | 0.00 | 0.058 | 4 | 1.11 | 1 | 2.13 | 0.459 | 2 | 1.33 | 3 | 1.16 | 0.61 | 1 | 1.56 | 4 | 1.16 | 0.576 |
 T790M | 3 | 0.74 | 3 | 1.29 | 0 | 0.00 | 0.183 | 1 | 0.28 | 2 | 4.26 | 0.036 | 2 | 1.33 | 1 | 0.39 | 0.31 | 1 | 1.56 | 2 | 0.58 | 0.401 |
 L747S | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 1 | 0.28 | 0 | 0.00 | 0.885 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
Combination of sensitizing and resistance mutations | ||||||||||||||||||||||
 G719X + S768I | 68 | 16.67 | 56 | 24.14 | 12 | 6.82 | 0.000 | 61 | 16.90 | 7 | 14.89 | 0.729 | 30 | 20.00 | 38 | 14.73 | 0.17 | 14 | 21.88 | 54 | 15.70 | 0.223 |
 S768I + L858R | 4 | 0.98 | 3 | 1.29 | 1 | 0.57 | 0.421 | 4 | 1.11 | 0 | 0.00 | 0.612 | 2 | 1.33 | 2 | 0.78 | 0.47 | 1 | 1.56 | 3 | 0.87 | 0.496 |
 19-del + T790M | 3 | 0.74 | 1 | 0.43 | 2 | 1.14 | 0.397 | 2 | 0.55 | 1 | 2.13 | 0.308 | 2 | 1.33 | 1 | 0.39 | 0.31 | 1 | 1.56 | 2 | 0.58 | 0.401 |
 L858R + T790M | 3 | 0.74 | 1 | 0.43 | 2 | 1.14 | 0.397 | 3 | 0.83 | 0 | 0.00 | 0.692 | 0 | 0.00 | 3 | 1.16 | 0.25 | 0 | 0.00 | 3 | 0.87 | 0.599 |
 19-del + S768I | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 G719X + S768I + L858R | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 1 | 1.56 | 0 | 0.00 | 0.157 |
 G719X + S768I + EGFR gene ampilification | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 L858R + 20INS | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 G719X + D761Y + EGFR gene ampilification | 1 | 0.25 | 1 | 0.43 | 0 | 0.00 | 0.569 | 1 | 0.28 | 0 | 0.00 | 0.885 | 1 | 0.67 | 0 | 0.00 | 0.37 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 19-del + T790M + C797S | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 0 | 0.00 | 1 | 2.13 | 0.115 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 G719X + S768I + T790M | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 0 | 0.00 | 1 | 2.13 | 0.115 | 0 | 0.00 | 1 | 0.39 | 0.63 | 0 | 0.00 | 1 | 0.29 | 0.843 |
 L858R + T790M + EGFR gene ampilification | 1 | 0.25 | 0 | 0.00 | 1 | 0.57 | 0.431 | 0 | 0.00 | 1 | 2.13 | 0.115 | 1 | 0.67 | 0 | 0.00 | 0.37 | 0 | 0.00 | 1 | 0.29 | 0.843 |
No EGFR targeted therapies mutations | ||||||||||||||||||||||
 V769fs,S1042I,H773L,V774M,G983W,D1127fs,R973Q,I926L,A1118V | 8 | 1.96 | 5 | 2.16 | 3 | 1.70 | 0.521 | 8 | 2.22 | 0 | 0.00 | 0.372 | 4 | 2.67 | 4 | 1.55 | 0.68 | 1 | 1.56 | 7 | 2.03 | 0.802 |