Fig. 2

Effect of BPA in the presence of S-9 mix on the flocculation of DNMT yeast. A DNMT yeast was grown at the indicated concentrations of BPA to OD600 of 3.3-3.7 in the presence of S-9 mix. The pictures were taken after allowing the agitated culture to settle at room temperature for 30 min. This experiment for measuring relative flocculation activity was repeated at least three times with similar results and the data from one representative experiment is shown. The histogram shows the relative flocculation activity. The data are presented as the means ± SEM from more than three independent experiments. Statistical analysis was performed using ANOVA, followed by Dunnett’s post hoc test. (*P < 0.05 versus control). B DNMT yeast was grown at the indicated concentrations of BPA (± S-9 mix) or MBP. Total RNA was extracted from the yeast cells, and the mRNA levels of FLO1 and ACT1 were determined by semi-quantitative RT-PCR. The results are representative of at least three independent experiments. The histograms show the relative intensity of FLO1 mRNA levels normalized to the levels of ACT1 mRNA and the control was set as 1.0. The data are presented as the means ± SEM from more than three independent experiments. Statistical analysis was performed using ANOVA, followed by Dunnett’s post hoc test. (*P < 0.05, **P < 0.01 versus control).