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Fig. 3 | Genes and Environment

Fig. 3

From: MicroRNA miR-627-5p restrains pulmonary artery smooth muscle cell dysfunction by targeting MAP 2 K4 and PI3K/AKT signaling

Fig. 3

MAP2K4 directly targets miR-627-5p. A The downstream targets of miR-627-5p were predicted using miRDB database and the top eight were chosen for following qRT-PCR analysis. B qRT-PCR analysis of candidate gene expression in PASMCs after miR-627-5p overexpression. C Western blotting analysis of MAP2K4 protein level in PASMCs after overexpressing miR-627-5p. D The binding site of miR-627-5p on MAP2K4 3’UTR was predicted at TargetScan website. E-F The luciferase activities of pmiR-RB-REPORT-MAP2K4–3′UTR-Wt/Mut vectors in PASMCs after miR-627-5p overexpression were assessed through luciferase reporter assay. ***p<0.001

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Genes and Environment

ISSN: 1880-7062

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