Fig. 5
From: Targeting the MALAT1 gene with the CRISPR/Cas9 technique in prostate cancer
A, B MALAT1 expression in DU-145 control cells and the transfected cells. C, D lncRNA MALAT1 is highly expressed in human PC cell carcinoma. E Products of RT-PCR in DU-145 transfected cells and non-transfected cells for expression appraise of MALAT1 gene on the agarose gel. M: molecular marker of DNA, 1: amplified fragments of MALAT1 [442 bp] in DU-145 transfected cells, 2: MALAT1 expression [1133 bp] in DU-145 non-transfected cells [control cells]. G LncRNA MALAT1 increases the proliferation of PC cell lines. Norma MALAT1 and knockdown-MALAT1 were transfected into DU-145 cells. The knockdown efficiency of MALAT1 was detected by RT-PCR. H DU-145 cells expressing MALAT1 cells and Normal MALAT1 were cultured in 96-well plates, and cell viability was measured using CCK-8 after 1, 3, and 5 days of culture. *P < 0.05, **P < 0.01, ***P < 0.001