Table 1 List of primers along with target gene and product length utilized in this research
From: Targeting the MALAT1 gene with the CRISPR/Cas9 technique in prostate cancer
Gene name | Primer sequence | Annealing temperature | Product length | Gene type |
|---|---|---|---|---|
BAX | F: 5′-AGGTCTTTTTCCGAGTGGCAGC-3’ | 64 °C | 234 bp | Proapoptosis |
R: 5′-GCGTCCCAAAGTAGGAGAGGAG-3’ | ||||
FAS | F: 5′-CAATTCTGCCATAAGCCCTGTC-3’ | 64 °C | 163 bp | Proapoptosis |
R: 5′-GTCCTTCATCACACAATCTACATCTTC-3’ | ||||
P53 | F: 5′-TGCGTGTGGAGTATTTGGATGAC-3’ | 64 °C | 170 bp | Proapoptosis |
R: 5′-CAGTGTGATGATGGTGAGGATGG-3’ | ||||
SURVIVIN | F: 5′-AGAACTGGCCCTTCTTGGAGG-3’ | 64 °C | 170 bp | Antiapoptosis |
R: 5′-CTTTTTATGTTCCTCTATGGGGTC-3’ | ||||
BCL2 | F: 5′-GACGACTTCTCCCGCCGCTAC-3’ | 64 °C | 245 bp | Antiapoptosis |
R: 5′-CGGTTCAGGTACTCAGTCATCCAC-3’ | ||||
GAPDH | F: 5′- GAAGGTGAAGGTCGGAGTC-3’ | 64 °C | 226 bp | Endogenous control |
R: 5′- GAAGATGGTGATGGGATTC-3’ |