In vivo genotoxicity assessment of a multiwalled carbon nanotube in a mouse ex vivo culture

Horibata, Katsuyoshi; Takasawa, Hironao; Hojo, Motoki; Taquahashi, Yuhji; Shigano, Miyuki; Yokota, Satoshi; Kobayashi, Norihiro; Sugiyama, Kei-ichi; Honma, Masamitsu; Hamada, Shuichi

doi:10.1186/s41021-022-00253-2

Genes and Environment

Table 1 Summary of in vivo genotoxicities and experimental conditions in MWNT-7-studies

From: In vivo genotoxicity assessment of a multiwalled carbon nanotube in a mouse ex vivo culture

Results	Exposure route	Genotoxicity test(s)	Tissue	Species	Treatment Regime^e	Timings of Dissection	References
Positive	whole-body inhalation^a	ex vivo MN assay^b	lung	mice	2 mg/m³, 2 h/d, 5 d	5 days after the final treatment	in this study
	intratracheal instillation	ex vivo MN assay^b	lung	mice	0.05 mg/animal	24 h after the single treatment	in this study
	inhalation exposure	ex vivo MN assay	lung	mice	10.8 mg/m³, 4 h/d, 4 d	72 h after the inhalation exposure	[9]
	inhalation exposure	comet assay	lung	mice	8.2 mg/m³, 4 h/d, 4 d	24 h after the inhalation exposure	[9]
	pharyngeal instillation	comet assay	lung	mice	<200 μg/mouse	24 h after the single pharyngeal aspiration	[9]
	intratracheal instillation	comet assay	lung	mice	<162 μg/mouse	1 day after the single treatment	[8]
	intratracheal instillation	comet assay	lung	mice	<0.2 mg/animal	3h after the single administration	[7]
	intratracheal instillation	gpt assay^c	lung	mice	<0.2 mg/animal/week, 4 w	8–12 weeks after the administrations	[7]
Negative	intratracheal instillation	lacZ assay^d	lung	mice	<109 μg/mouse/week, 4 w	60 days after the final treatment	[10]
	intratracheal instillation	comet assay	lung	mice	<109 μg/mouse/week, 4 w	60 days after the final treatment	[10]
	intratracheal instillation	gpt assay^c	lung	rats	<1 mg/kg	4 weeks after the single administration	[11]
	intratracheal instillation	Pig-a assay	eryth.^f	rats	<1 mg/kg	4 weeks after the single administration	[11]
	intratracheal instillation	MN test	eryth.^f	mice	0.05 mg/animal	24h after the single treatment	in this study
	oral gavage	MN test	b.m.^g	mice	<20 mg/kg/d, 2d	24 h after the final administration	[12]
	inhalation exposure	γ-H2AX	eryth.^f, lung	mice	8.2 mg/m³, 4 h/d, 4 d	24 h after the exposure	[9]
	inhalation exposure	MN test	b.m.^g	mice	8.2 mg/m³, 4 h/d, 4 d	24 h after the exposure	[9]

^awhole-body inhalation exposure of well-dispersed aerosol of Taquann-treated MWCNTs (T-CNTs) [13].
^bthe ex vivo micronucleus (MN) assay was performed according to the method described by Lindberg HK, et al. [14].
^c transgenic rodent gene mutation assay using gpt-delta transgenic animals
^dtransgenic rodent gene mutation assay using MutaTM mice
^emaximum dose in each report shown with “<”
^f eryth. erythrocytes
^g b.m. bone marrow

Back to article page

ISSN: 1880-7062

Contact us

Submission enquiries: Access here and click Contact Us
General enquiries: info@biomedcentral.com