Fig. 6From: Easily-controllable, helper phage-free single-stranded phagemid production systemSchematic diagram of the experimental procedures for the production of phages containing the ss phagemid of interest and the DNA purification on an anion-exchange column. Day 1, Transformation of E. coli bearing VCSM13ΔPS(PBAD-pII) with phagemid of interest. Day 2, Pre-culture of a single colony in LB medium containing optimal antibiotics and glucose. Day 3, Culture in 2 × YT medium and induce phage production by arabinose. Day 4, Precipitation of the phage and purification of the ss phagemid on an anion-exchange columnBack to article page