Fig. 5

Functional complementation of KsgA rRNA methyltransferase activity by KsgA (1-213) in WT (AB1157) and ksgA-deficient (AB1157 ksgA) E. coli strains. (a) Procedure of measuring kasugamycin-resistance. Each strain of E.coli was grown overnight at 37 °C in LB medium containing antibiotics. 10²-times diluted overnight culture was incubated at 37 °C to an OD₆₀₀ of 0.2 and then diluted 20-times in LB medium containing IPTG and kasugamycin. After incubation at 37 °C for 15 h, the growth of cells was estimated by measuring OD₆₀₀. (b) The result of kasugamycin assay. Mean + S.E. values of three independent experiments are shown (n = 3). Two asterisks (**) indicates p values < 0.01