Fig. 5From: The H2TH-like motif of the Escherichia coli multifunctional protein KsgA is required for DNA binding involved in DNA repair and the suppression of mutation frequenciesFunctional complementation of KsgA rRNA methyltransferase activity by KsgA (1-213) in WT (AB1157) and ksgA-deficient (AB1157 ksgA) E. coli strains. (a) Procedure of measuring kasugamycin-resistance. Each strain of E.coli was grown overnight at 37 °C in LB medium containing antibiotics. 102-times diluted overnight culture was incubated at 37 °C to an OD600 of 0.2 and then diluted 20-times in LB medium containing IPTG and kasugamycin. After incubation at 37 °C for 15 h, the growth of cells was estimated by measuring OD600. (b) The result of kasugamycin assay. Mean + S.E. values of three independent experiments are shown (n = 3). Two asterisks (**) indicates p values < 0.01Back to article page