Fig. 2
Detection of apoptosis by the AO-staining method. The wing discs were dissected from wild-type larvae (mwh/flr) that were irradiated with polychromatic UV light (peak at 312 nm) at 10 kJ/m2 according to the time schedule shown in (A). The dissected discs were soaked in Drosophila Ringer’s solution containing AO for a few minutes, then the fluorescent spots were observed (B). The indicated times are the time periods for which the larvae were kept in the dark after irradiation was stopped