Fig. 7

Apoptosis and mutation induced by monochromatic UV light in wild-type Drosophila (mwh/flr). Apoptosis was determined by the number of clusters stained with AO. (A) The profiles of apoptosis induced by monochromatic UV irradiation with 310-nm light at 14 kJ/m² (closed circles), 320-nm light at 305 kJ/m² (open circles), 330-nm light at 419 kJ/m² (closed triangles), 340-nm light at 406 kJ/m² (open triangles), 360-nm light at 477 kJ/m² (closed diamonds), or no irradiation (cross). (B) The relationship between the number of AO-stained clusters per wing and total spots per wing at each wavelength (symbols are the same as in panel (A)). The values of R² were obtained by a linear approximation method. (C) The action spectra of the mutagenicity were determined as the total spots per wing normalized by the UV fluence (open circles) as described previously [47], and apoptosis inducibility was determined as the number of clusters per wing disc at 6 h normalized by the UV fluence (closed circle)