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Fig. 3 | Genes and Environment

Fig. 3

From: Development of a micronucleus test using the EpiAirway™ organotypic human airway model

Fig. 3

Changes in transepithelial electrical resistance and tissue morphology induced in various experimental conditions. a Schematic of transepithelial electrical resistance (TEER) measurement and histology of EpiAirway™ tissue under various experimental conditions. Group 1, immediately after a 3-day acclimatization period; Group 2, no test chemical treatment, cytochalasin B (CB) incubation, or epidermal growth factor (EGF) simulation throughout the experimental period; Group 3, solvent treatment; Group 4, no chemical treatment followed by EGF stimulation only; Group 5, no chemical treatment followed by CB incubation only; and Group 6, solvent treatment followed by CB incubation and EGF stimulation. b Tissue integrity assay. Values are means ± standard deviation of triplicate inserts. *Significantly different from the ‘Group 2’ (p < 0.05, Welch’s t-test). c Representative images of tissue sections stained with hematoxylin and eosin from samples collected on Day 0 and Day 4

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