Fig. 5
From: Development of a micronucleus test using the EpiAirway™ organotypic human airway model
Percentage of binucleated cells and frequency of micronucleated binucleated cells by test chemical treatment. a Schematic of test chemical treatment followed by cytochalasin B (CB) incubation and epidermal growth factor (EGF) stimulation. b, c, and d Percentage of binucleated cells (%BNCs) and frequency of micronucleated binucleated cells (%MN) after apical treatment with (b) mitomycin C (MMC), (c) methyl methanesulfonate (MMS), and (d) colchicine (COL) in the EpiAirway™ model. Results are plotted with a blue line indicating %BNCs (left axis) and a red line indicating %MN (right axis). %BNCs are means ± standard deviations of triplicate inserts, while %MN is the combined value of triplicate inserts. *Significantly different from the solvent control group (p < 0.05, Fisher’s exact test)