Table 2 Mutant frequencies in the lungs of MutaMouse given styrene [Male mice were dosed once daily for 28Â days (Oral administration, tissues were collected 3Â days after the final administration)]
From: In vivo mutagenicity assessment of styrene in MutaMouse liver and lung
Substance | Dose (mg/kg/day, p.o.) | Animal ID No | Number of plaque | Number of packaging | Number of mutants | Mutant frequency (10−6) | Group Mean ± S.D. (× 10−6) |
|---|---|---|---|---|---|---|---|
Corn oil | 0 | 3001 | 313,200 | 1 | 7 | 22.3 | 33.4 ± 9.7 |
3002 | 396,000 | 1 | 10 | 25.3 | |||
3003 | 620,100 | 1 | 25 | 40.3 | |||
3004 | 383,400 | 1 | 13 | 33.9 | |||
3005 | 486,000 | 1 | 22 | 45.3 | |||
Styrene | 75 | 3101 | 569,700 | 1 | 27 | 47.4 | 108.6 ± 130.5 * (55.6 ± 16.4) # |
3102 | 899,100 | 1 | 45 | 50.1 | |||
3103 | 830,700 | 1 | 310 | 373.2 | |||
3104 | 680,400 | 1 | 51 | 75.0 | |||
3105 | 531,000 | 1 | 19 | 35.8 | |||
3106 | 543,600 | 1 | 38 | 69.9 | |||
150 | 3201 | 620,100 | 1 | 34 | 54.8 | 46.1 ± 20.1 | |
3202 | 368,100 | 1 | 27 | 73.3 | |||
3203 | 854,100 | 1 | 25 | 29.3 | |||
3204 | 522,000 | 1 | 26 | 49.8 | |||
3205 | 594,900 | 1 | 14 | 23.5 | |||
300 | 3301 | 649,800 | 1 | 28 | 43.1 | 43.7 ± 6.1 | |
3302 | 547,200 | 1 | 21 | 38.4 | |||
3303 | 537,300 | 1 | 20 | 37.2 | |||
3304 | 927,000 | 1 | 47 | 50.7 | |||
3305 | 713,700 | 1 | 35 | 49.0 | |||
ENU | 100 | 3401 | 737,100 | 1 | 123 | 166.9 | 180.7 ± 35.0 ** |
3402 | 482,400 | 1 | 61 | 126.5 | |||
3403 | 670,500 | 1 | 130 | 193.9 | |||
3404 | 763,200 | 1 | 153 | 200.5 | |||
3405 | 575,100 | 1 | 124 | 215.6 |