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Fig. 5 | Genes and Environment

Fig. 5

From: Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids

Fig. 5

Responses of reporter gene assay yeast strains expressing ERα and ERβ (W303a and cwp1Δcwp2Δ) to known antagonist ligands. ERα and ERβ assay yeasts were exposed to ICI (A and C), Tam (B and D), Ral (C), and MPP (F). Antagonistic activity (A and B) and partial agonist activity (C-F) were measured. Regarding ICI and MPP, inverse agonist activity was also measured: ICI (G) and Tam (H). CYC yeasts (W303a and cwp1Δcwp2Δ) that constitutively express β-gal were used as references in antagonist and inverse agonist assays. Data represent the mean ± SD of triplicate experiments

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Genes and Environment

ISSN: 1880-7062

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