Skip to main content
Fig. 4 | Genes and Environment

Fig. 4

From: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Fig. 4

DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase ζ (panel A), DNA polymerase η (panel B) and Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) (panel C). DNA polymerase ζ (1.7 ng), DNA polymerase η (0.4 ng) and Dpo4 (0.4 ng) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls

Back to article page

Genes and Environment

ISSN: 1880-7062

Contact us