Fig. 1From: Short-term in vivo testing to discriminate genotoxic carcinogens from non-genotoxic carcinogens and non-carcinogens using next-generation RNA sequencing, DNA microarray, and qPCRCluster analysis of gene expression in mouse liver after DEN treatment quantified by qPCR. The expression of 50 genes was clustered by hierarchical clustering after DEN treatment. Results of 4 h and 28 days after a single shot were analyzed separately. The color displays show the log2 (expression ratio) as (1) red when the treatment sample is up-regulated relative to the control sample, (2) blue when the treatment sample is down-regulated relative to the control sample, and (3) white when the log2 (expression ratio) is close to zero [40]. At 4 h, all 20 Grp 1 genes showed a dose-dependent increase of more than 3–64-fold. Twelve Grp 2 genes were suggested to have a gradual dose-dependent increase of less than that for the expression in Grp1. Two Grp 4 genes exhibited a dose-dependent decrease of less than 0.3-fold. Fifteen Grp 3 genes showed few changes in gene expression. At 28 days, three Grp 1 genes showed a dose-dependent increase of more than four-fold. Seventeen Grp 2 genes were suggested to have a gradual dose-dependent increase, though less than that for the expression in Grp 1. Ungrouped Igfbp1 showed a dose-dependent decrease of less than 0.3-fold. 22 Grp 3 genes showed fewer changes in gene expressionBack to article page