| control | NDMA (µmol/dish) | NDEA (µmol/dish) | ||
---|---|---|---|---|---|
 | 0 | 10 | 30 | 30 | 80 |
Apoptosis (number of clusters stained with AO/disc) | |||||
Time post-treatment (h) | Â | Â | Â | Â | Â |
  0 | 14.8 ±  1.7 |  29.7 ± 17.1 |  42.9 ± 16.8 | 24.9 ± 11.1 |  41.1 ± 21.1 |
  6 | 14.4 ±  7.1 |  79.1 ± 37.9 | 112.1 ± 52.2 | 45.9 ± 18.9 |  76.8 ± 26.8 |
  12 | 21.0 ± 12.5 | 154.7 ± 70.0 | 244.8 ± 56.4 | 51.8 ±  9.6 | 129.0 ± 35.4 |
  18 | 15.6 ±  8.4 | 188.9 ± 61.7 | 170.9 ± 61.5 | 71.9 ± 36.0 | 146.4 ± 46.1 |
  24 | 23.5 ± 10.1 |  60.6 ± 27.4 | 141.9 ± 40.4 | 38.1 ±  9.0 | 153.4 ± 36.6 |
Mutation (number of total spots/wing) | |||||
 | 0.53 | 5.39** | 10.6** | 2.13** | 3.35** |
Survival (%) | |||||
 | 100 | 134 | 94.6 | 92.2 | 52.7 |