Polymorphism in autophagy-related genes LRP1 and CAPZA1 may promote gastric mucosal atrophy

Genes and Environment

Table 2 Probe sequence and melting temperatures of probe-based HRM

Gene	SNP	Genotyping	Probe-based HRM
Gene	SNP	Genotyping	Probe sequence (5‘to 3’)	Melting temperature (℃)
LRP1	rs715948	Probe-based HRM	AGGGGAGAGGCTAAATGTGGAGCCACCATAT
	rs1799986	Probe-based HRM	AGGACTGCATGGACGGCTCAGATGAGATT	50→95
	rs1800137	Probe-based HRM	CCGCCAGACCATCATCCAGGGCATCGCA	50→95
	rs1800159	Probe-based HRM	AACACCTGCTCTGTCCTAGTGTCCTCATGCGAA	50→95
	rs11172113	Probe-based HRM	AGGAAAGAGCCACTGGGCAACACCCAAAATAGTT	50→95
	rs11837145	Probe-based HRM	CCCCACACTTCTGTTCATTGGGTTAGATTTTACA	50→95
CAPZA1	rs4423118	Probe-based HRM	TCACCTCCACATGCTGAGGTCAATACTGGATATC	50→95
	rs4525162	Probe-based HRM	CATTACATTTATGCTTCCAAGGAATCAATTGTCGCA	50→95
	rs10885188	Probe-based HRM	TCTCCTGAGAGAGACGCAATGAGAAGTTTATCGC	50→95
	rs58618380	Probe-based HRM	TGGCTGAAAGAACAATTGTTTTCCAGTTTCACGA	50→95
	rs71481135	Probe-based HRM	AAATAAAGCAATTCCTCATGCTGCCATGGCCGGC	50→95
LAMP1	rs9577230	Probe-based HRM	TCCAGTTGAATACAATTCTTCCTGACGCCAAGC	50→95
	rs9577502	Probe-based HRM	GACATAGTAAGATCTTATCTCTTTTTAAAAAAAAAAAAAATAC	50→95
	rs12871648	Probe-based HRM	TTGTTGAATGAATCAGAGGACTGCCTGACTCGAT	50→95

ISSN: 1880-7062