Fig. 2

Representative electropherograms for the missense and novel TP53 mutations in Rwandan patients with gastric cancer. A: p.R282G missense TP53 mutation detected in case GC035. The lower electropherogram shows a g.7,673,776G>C (c.844C>G) TP53 mutation corresponds to a missense mutation on codon 282, which is associated with p.R282G. The upper electropherogram is derived from a control case without TP53 mutation. B: p.E286V missense TP53 mutation detected in case GC089. The lower electropherogram shows a g.7,673,763T>A (c.857A>T) TP53 mutation corresponds to a missense mutation on codon 286, which is associated with p.E286V. The upper electropherogram is derived from a control case without TP53 mutation. C: A frameshift TP53 mutation (g.7,675,139_7,675,157delCGGACGCGGGTGCCGGGCG corresponding to c.459_477delCGGCACCCGCGTCCGCGCC), which is associated with p.G154Wfs*10, was newly identified in this study. The middle and lower electropherograms are the results of gastric carcinoma containing this novel mutation in case GC021. In the middle electropherogram, mixed peaks are seen as result of the deletion of 19 bases. The lower electropherogram shows the TP53 mutation sequence after TA subcloning. The shaded area represents 19 bases deleted. The upper electropherogram corresponds to the wild-type sequence (control case)