Fig. 1

Astaxanthin inhibits C666-1 cell growth while augmenting cell cycle arrest and apoptosis. A. The impacts of different concentrations of astaxanthin (1, 10, 20 mg/mL) on viable C66-1 cells were evaluated by CCK-8 assays, and 10 mg/ml astaxanthin was identified for subsequent experiments. B. The OD values of C666-1 cells in control and astaxanthin groups were measured by CCK-8 assays. C. C666-1 cell proliferation in control or astaxanthin group was measured by colony forming assays. D-E. The influence of astaxanthin on C666-1 cell apoptotic rate (D) and cell cycle progression (E) were detected using flow cytometry. ^*p < 0.05, ^**p < 0.01, ^##p < 0.01