Table 1 Nucleotide sequences of primers used and amplification conditions
Gene | Primer | Sequence | Amplification profile Temperature °C/time (sec) |  | Product size, bp | Restriction Enzyme | Cut Product size, bp | ||
|---|---|---|---|---|---|---|---|---|---|
D | A | E | C | ||||||
TLR4 | 299TLR4F | 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′ | 94 (30) | 55 (30) | 72 (30) | 35 | 249 | Nco1 (Fermentas) | W = 249 |
299TLR4R | 5-GATCAACTTCTGAAAAAGCATTCCCAC-3′ | M = 223 + 26 | |||||||
399TLR4F | 5′-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA-3′ | 94 (30) | 55 (30) | 72 (30) | 35 | 406 | Hinf1 (Fermentas) | W = 406 | |
399TLR4R | 5′-ACCTGAAGACTGGAGAGTGAGTTAAATGCT-3′ | M = 377 + 29 | |||||||