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Table 1 Nucleotide sequences of primers used and amplification conditions

From: Toll-like receptor (TLR4) Asp299Gly and Thr399Ile polymorphisms in relation to clinical falciparum malaria among Nigerian children: a multisite cross-sectional immunogenetic study in Lagos

Gene Primer Sequence Amplification profile Temperature °C/time (sec)   Product size, bp Restriction Enzyme Cut Product size, bp
D A E C
TLR4 299TLR4F 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′ 94 (30) 55 (30) 72 (30) 35 249 Nco1 (Fermentas) W = 249
299TLR4R 5-GATCAACTTCTGAAAAAGCATTCCCAC-3′ M = 223 + 26
399TLR4F 5′-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA-3′ 94 (30) 55 (30) 72 (30) 35 406 Hinf1 (Fermentas) W = 406
399TLR4R 5′-ACCTGAAGACTGGAGAGTGAGTTAAATGCT-3′ M = 377 + 29