Articles

Advances in neural organoid systems and their application in neurotoxicity testing of environmental chemicals

Due to the complex structure and function of central nervous system (CNS), human CNS in vitro modeling is still a great challenge. Neurotoxicity testing of environmental chemicals mainly depends on the traditi...

Authors: Yuanyuan Zheng, Fangrong Zhang, Shengmin Xu and Lijun Wu

Mutagenicity of carcinogenic heterocyclic amines in Salmonella typhimurium YG strains and transgenic rodents including gpt delta

Chemical carcinogens to humans have been usually identified by epidemiological studies on the relationships between occupational or environmental exposure to the agents and specific cancer induction. In contra...

Authors: Takehiko Nohmi and Masahiko Watanabe

The effect of aging on the repeated-dose liver micronucleus assay

The liver micronucleus (MN) assay is an effective and important in vivo test for detecting genotoxic compounds. In particular, the repeated-dose liver MN (RDLMN) assay which greatly facilitates incorporation o...

Authors: Miyuki Shigano, Hironao Takasawa and Shuichi Hamada

High resolution melting analysis and detection of Leishmania resistance: the role of multi drug resistance 1 gene

Pentavalent antimonial compounds are currently used to treat leishmaniasis and resistance to these drugs is a serious problem. Multidrug resistance protein is an efflux pump of the cell membrane that expels fo...

Authors: Maryam Fekri Soofi Abadi, Alireza Moradabadi, Reza Vahidi, Saeedeh Shojaeepour, Sara Rostami, Iman Rad and Shahriar Dabiri

PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40

Peroxiredoxin 1 (PRDX1) is a member of a ubiquitous family of thiol peroxidases that catalyze the reduction of peroxides, including hydrogen peroxide. It functions as an antioxidant enzyme, similar to catalase...

Authors: Takahito Moriwaki, Akari Yoshimura, Yuki Tamari, Hiroyuki Sasanuma, Shunichi Takeda, Masayuki Seki and Keizo Tano

miR221 regulates cell migration by targeting annexin a1 expression in human mesothelial MeT-5A cells neoplastic-like transformed by multi-walled carbon nanotube

Multi-walled carbon nanotube (MWCNT) is one of the most widely used manufactured nanomaterials, however, its potential harmful effect on human health is of great concern. Previously we have shown the acute and...

Authors: Li Ju, Lijin Zhu, Hao Wu, Min Yu, Xianhong Yin, Zhenyu Jia, Lingfang Feng, Shibo Ying, Hailing Xia, Shuzhi Zhang, Jianlin Lou and Jun Yang

Dietary heterocyclic aromatic amine intake and cancer risk: epidemiological evidence from Japanese studies

Heterocyclic aromatic amines (HAAs), which are formed from the reaction of creatine or creatinine, amino acids, and sugars in meat and fish cooked at high temperatures, have been shown to be mutagenic in bacte...

Authors: Motoki Iwasaki and Shoichiro Tsugane

Gene expression analysis in NSAID-induced rat small intestinal disease model with the intervention of berberine by the liquid chip technology

Investigate the effect and mechanism of berberine on the small intestinal mucosa of non-steroidal anti-inflammatory drugs (NSAIDs) related small intestinal injury.

Authors: Guanqun Chao, Qianqian Wang, Fangxu Ye and Shuo Zhang

Achievements and memories of Dr. Takashi Sugimura

Authors: Keiji Wakabayashi

In vivo and in vitro mutagenicity of perillaldehyde and cinnamaldehyde

Perillaldehyde and cinnamaldehyde are natural substances found in plants that are used as flavoring ingredients. Due to the α,β-unsaturated aldehydes in their structures, these compounds are expected to be DNA...

Authors: Masamitsu Honma, Masami Yamada, Manabu Yasui, Katsuyoshi Horibata, Kei-ichi Sugiyama and Kenichi Masumura

Metabolism and biomarkers of heterocyclic aromatic amines in humans

Heterocyclic aromatic amines (HAAs) form during the high-temperature cooking of meats, poultry, and fish. Some HAAs also arise during the combustion of tobacco. HAAs are multisite carcinogens in rodents, induc...

Authors: Medjda Bellamri, Scott J. Walmsley and Robert J. Turesky

Free radical-mediated acetaldehyde formation by model reactions of dietary components: effects of meat, wine, cooking oil and coffee

Alcohol consumption and the ingestion of red meat and oxidized cooking oil are risk factors of gastric and colorectal cancers. We reported that acetaldehyde (AcAld) is generated from Heme/Mb/Meat-Linoleate-EtO...

Authors: Hiroshi Kasai and Kazuaki Kawai

Characteristic mutations induced in the small intestine of Msh2-knockout gpt delta mice

Base pair mismatches in genomic DNA can result in mutagenesis, and consequently in tumorigenesis. To investigate how mismatch repair deficiency increases mutagenicity under oxidative stress, we examined the ty...

Authors: Yasunobu Aoki, Mizuki Ohno, Michiyo Matsumoto, Michi Matsumoto, Kenichi Masumura, Takehiko Nohmi and Teruhisa Tsuzuki

Transcription factor 7-like 2 gene- smoking interaction on the risk of diabetic nephropathy in Chinese Han population

To evaluate the relationship between transcription factor 7-like 2 (TCF7L2) gene polymorphism and diabetic nephropathy (DN) risk, as well as the effect of gene-environment interactions on DN risk in Chinese Han p...

Authors: Peng Xue, Haihong Cao, Zhimin Ma, Ying Zhou and Nian Wang

New homozygous gpt delta transgenic rat strain improves an efficiency of the in vivo mutagenicity assay

Gene mutation assays in transgenic rodents are useful tools to investigate in vivo mutagenicity in a target tissue. Using a lambda EG10 transgene containing reporter genes, gpt delta transgenic mice and rats have...

Authors: Kenichi Masumura, Tomoko Ando, Akiko Ukai, Sho Fujiwara, Shigeo Yokose, Xinyue You, Takayoshi Suzuki, Hiroyuki Hayashi, Takehiko Nohmi, Hisayoshi Takagi and Masamitsu Honma

Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides

Etheno (and ethano) derivatives of nucleic acid bases have an extra 5-membered ring attached. These were first noted as wyosine bases in tRNAs. Some were fluorescent, and the development of etheno derivatives ...

Authors: F. Peter Guengerich and Pratibha P. Ghodke

Dynamic assessing silica particle-induced pulmonary fibrosis and associated regulation of long non-coding RNA expression in Wistar rats

Exposure to respirable crystalline silica (RCS) can induce accelerated silicosis (AS), a form of silicosis that is more progressive and severe form of silicosis. In this project we aimed to assess processes of...

Authors: Linlin Sai, Xuejie Qi, Gongchang Yu, Juan Zhang, Yuxin Zheng, Qiang Jia and Cheng Peng

Prediction of gene expression regulation by human microRNAs in Plasmodium falciparum

Malaria is a disease annually causing over 400,000 deaths. Deep understanding of molecular and genetic processes underlying its life cycle and pathogenicity is required to efficiently resist it. RNA interferen...

Authors: Alexandr Grinev, Natalya Fokina, Denis Bogomolov, Iza Berechikidze and Yuliya Lazareva

MicroRNAs as the critical regulators of cisplatin resistance in gastric tumor cells

Combined chemotherapeutic treatment is the method of choice for advanced and metastatic gastric tumors. However, resistance to chemotherapeutic agents is one of the main challenges for the efficient gastric ca...

Authors: Amir Sadra Zangouei and Meysam Moghbeli

The role of heterocyclic aromatic amines in colorectal cancer: the evidence from epidemiologic studies

Since Dr. Sugimura’s discovery of heterocyclic aromatic amines (HAA) in broiled fish, many epidemiological studies have been conducted to investigate their role in human cancers, often focusing on colorectal c...

Authors: Loïc Le Marchand

Vitamin D receptor gene polymorphisms and its interactions with environmental factors on renal cell carcinoma risk

We designed a case-control study to investigate the effect of vitamin D receptor gene (VDR) gene single nucleotide polymorphisms (SNPs) and possible gene- environment interaction on the susceptibility of renal...

Authors: Tian Jianhai, Lv Jian, Zhang Long, Wang Wei, Zhang Shumao, Wang Yiming and Li Xiaojuan

Study of the probable genotoxic effects of Zolone (Phosalone) exposure in mice bone marrow derived cells

Approximately, 2 million tonnes of pesticides are utilized annually worldwide. Phosalone (Pln), an organophosphorus pesticide, acts as an insecticide and acaricide to control pests of crops such as nuts, citru...

Authors: Zohre Khodabandeh, Mahmoud Etebari and Mehdi Aliomrani

Association of apolipoprotein B XbaI (rs693) polymorphism and gallstone disease risk based on a comprehensive analysis

Our aim was to investigate the association between XbaI gene polymorphisms in the apolipoprotein B (APOB) gene and gallstone disease (GD) risk through a comparison of the allele and genotype distribution frequenc...

Authors: Haifeng Zhu, Linhai Yu and Linsong Feng

Development of a new quantitative structure–activity relationship model for predicting Ames mutagenicity of food flavor chemicals using StarDrop™ auto-Modeller™

Food flavors are relatively low molecular weight chemicals with unique odor-related functional groups that may also be associated with mutagenicity. These chemicals are often difficult to test for mutagenicity...

Authors: Toshio Kasamatsu, Airi Kitazawa, Sumie Tajima, Masahiro Kaneko, Kei-ichi Sugiyama, Masami Yamada, Manabu Yasui, Kenichi Masumura, Katsuyoshi Horibata and Masamitsu Honma

New insights on CRISPR/Cas9-based therapy for breast Cancer

CRISPR/Cas9 has revolutionized genome-editing techniques in various biological fields including human cancer research. Cancer is a multi-step process that encompasses the accumulation of mutations that result ...

Authors: Hussein Sabit, Shaimaa Abdel-Ghany, Huseyin Tombuloglu, Emre Cevik, Amany Alqosaibi, Fatma Almulhim and Afnan Al-Muhanaa

Methylation as a critical epigenetic process during tumor progressions among Iranian population: an overview

Cancer is one of the main health challenges and leading causes of deaths in the world. Various environmental and genetic risk factors are associated with tumorigenesis. Epigenetic deregulations are also import...

Authors: Iman Akhlaghipour, Amir Reza Bina, Mohammad Reza Abbaszadegan and Meysam Moghbeli

Formaldehyde exposure and leukemia risk: a comprehensive review and network-based toxicogenomic approach

Formaldehyde is a widely used but highly reactive and toxic chemical. The International Agency for Research on Cancer classifies formaldehyde as a Group 1 carcinogen, based on nasopharyngeal cancer and leukemi...

Authors: Doo Seok Kang, Hyun Soo Kim, Jong-Hyeon Jung, Cheol Min Lee, Yeon-Soon Ahn and Young Rok Seo

Mass spectrometric profiling of DNA adducts in the human stomach associated with damage from environmental factors

A comprehensive understanding of DNA adducts, one of the most plausible origins of cancer mutations, is still elusive, especially in human tissues in clinical settings. Recent technological developments have f...

Authors: Ippei Ohnishi, Yuji Iwashita, Yuto Matsushita, Shunsuke Ohtsuka, Takashi Yamashita, Keisuke Inaba, Atsuko Fukazawa, Hideto Ochiai, Keigo Matsumoto, Nobuhito Kurono, Yoshitaka Matsushima, Hiroki Mori, Shioto Suzuki, Shohachi Suzuki, Fumihiko Tanioka and Haruhiko Sugimura

In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds

The in vivo comet assay is used to evaluate the genotoxic potential of compounds by detecting DNA strand breaks in cells isolated from animal tissue. The comet assay of hepatocytes is well established; however, t...

Authors: Haruna Tahara, Yoshinori Yamagiwa, Yu Haranosono and Masaaki Kurata

Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

The PIGRET assay is one of the Pig-a assays targeting reticulocytes (RETs), an in vivo genotoxicity evaluation method using flow cytometry with endogenous reporter glycosylphosphatidylinositol anchor protein. The...

Authors: Satsuki Chikura, Takafumi Kimoto, Satoru Itoh, Hisakazu Sanada, Shigeharu Muto and Katsuyoshi Horibata

Radio-adaptive response and correlation of non-homologous end joining repair gene polymorphisms [XRRC5 (3R/2R/1R/0R), XRCC6(C/G) and XRCC7 (G/T)] in human peripheral blood mononuclear cells exposed to gamma radiation

Radio-adaptive response (RAR) is transient phenomena, where cells conditioned with a small dose (priming) of ionizing radiation shows significantly reduced DNA damage with a subsequent high challenging dose. T...

Authors: Shridevi Shelke and Birajalaxmi Das

Cancer interception by interceptor molecules: mechanistic, preclinical and human translational studies with chlorophylls

Before ‘cancer interception’ was first advocated, ‘interceptor molecules’ had been conceived as a sub-category of preventive agents that interfered with the earliest initiation steps in carcinogenesis. Three d...

Authors: Roderick H. Dashwood

Weight of evidence approach using a TK gene mutation assay with human TK6 cells for follow-up of positive results in Ames tests: a collaborative study by MMS/JEMS

Conflicting results between bacterial mutagenicity tests (the Ames test) and mammalian carcinogenicity tests might be due to species differences in metabolism, genome structure, and DNA repair systems. Mutagen...

Authors: Manabu Yasui, Takayuki Fukuda, Akiko Ukai, Jiro Maniwa, Tadashi Imamura, Tsuneo Hashizume, Haruna Yamamoto, Kaori Shibuya, Kazunori Narumi, Yohei Fujiishi, Emiko Okada, Saori Fujishima, Mika Yamamoto, Naoko Otani, Maki Nakamura, Ryoichi Nishimura…

Correction to: Species diversity and molecular analysis of opportunistic Mycobacterium, Nocardia and Rhodococcus isolated from the hospital environment in a developing country, a potential resources for nosocomial infection

An amendment to this paper has been published and can be accessed via the original article.

Authors: Marzieh Siavashifar, Fatemeh Rezaei, Tahereh Motallebirad, Davood Azadi, Abdorrahim Absalan, Zahra Naserramezani, Mohadeseh Golshani, Morteza Jafarinia and Kazem Ghaffari

The ACE and ACTN3 polymorphisms in female soccer athletes

We investigated the association of ACE I/D and ACTN3 R577X polymorphisms with the performance of Chinese elite female soccer athletes for the first time.

Authors: Qi Wei

Effect of sampling time on somatic and germ cell mutations induced by acrylamide in gpt delta mice

Acrylamide (AA) is a rodent carcinogen and classified by the IARC into Group 2A (probable human carcinogen). AA has been reported to induce mutations in transgenic rodent gene mutation assays (TGR assays), the...

Authors: Soichiro Hagio, Naho Tsuji, Satoshi Furukawa, Kazuya Takeuchi, Seigo Hayashi, Yusuke Kuroda, Masamitsu Honma and Kenichi Masumura

Panel of significant risk factors predicts early stage gastric cancer and indication of poor prognostic association with pathogens and microsatellite stability

There are very few studies covering the epidemiological risk factors associated with Epstein Barr Virus (EBV) and Microsatellite stability for Gastric Cancer (GC) cases. Early diagnosis of GC through epidemiologi...

Authors: Payel Chakraborty, Souvik Ghatak, Saia Chenkual, Lalawmpuii Pachuau, John Zohmingthanga, Zothankima Bawihtlung, Lalfakzuala Khenglawt, Jeremy L. Pautu, Arindam Maitra, Lalchhandama Chhakchhuak and Nachimuthu Senthil Kumar

Species diversity and molecular analysis of opportunistic Mycobacterium, Nocardia and Rhodococcus isolated from the hospital environment in a developing country, a potential resources for nosocomial infection

Hospital environmental resources have a significant role in cross-transmission of opportunistic pathogens such as actinomycetes species to the patients. Actinomycetes have a remarkable capability to survive in...

Authors: Marzieh Siavashifar, Fatemeh Rezaei, Tahereh Motallebirad, Davood Azadi, Abdorrahim Absalan, Zahra Naserramezani, Mohadeseh Golshani, Morteza Jafarinia and Kazem Ghaffari

The effect of polymorphisms (174G> C and 572C> G) on the Interleukin-6 gene in coronary artery disease: a systematic review and meta-analysis

Coronary Artery Disease (CAD) is caused by the blockage of the coronary arteries. it is argued that there has an association between the Interleukin-6 gene and the occurrence of atherosclerosis, coronary arter...

Authors: Nader Salari, Kamran Mansouri, Amin Hosseinian-Far, Hooman Ghasemi, Masoud Mohammadi, Rostam Jalali and Aliakbar Vaisi-Raygani

In memory of Dr. Takashi Sugimura, 1926–2020

Authors: Masami Yamada

Screening for Ames mutagenicity of food flavor chemicals by (quantitative) structure-activity relationship

(Quantitative) Structure-Activity Relationship ((Q)SAR) is a promising approach to predict the potential adverse effects of chemicals based on their structure without performing toxicological studies. We evalu...

Authors: Masamitsu Honma, Airi Kitazawa, Toshio Kasamatsu and Kei-ichi Sugiyama

Report of the Joint Meeting of the 6th Asian Congress on Environmental Mutagens and the 48th Annual Meeting of the Japanese Environmental Mutagen Society, Tokyo, November 18–20, 2019

The 6th Asian Congress on Environmental Mutagens (ACEM) was held at Hitotsubashi Hall, Chiyoda City, Tokyo on November 18–20, 2019, in conjunction with the 48th Annual Meeting of the Japanese Environmental Mut...

Authors: Masamitsu Honma

Oxidation resistance 1 functions in the maintenance of cellular survival and genome stability in response to oxidative stress-independent DNA damage

DNA damage is generated by various intrinsic and extrinsic sources such as reactive oxygen species (ROS) and environmental mutagens, and causes genomic alterations. DNA damage response (DDR) is activated to in...

Authors: Ako Matsui, Kazunari Hashiguchi, Masao Suzuki and Qiu-Mei Zhang-Akiyama

New indicator Escherichia coli strain for rapid and accurate detection of supF mutations

The supF gene of Escherichia coli is useful for forward mutation analysis in bacterial and mammalian cells used in mutagenesis and DNA repair studies. Indicator E. coli strains, such as KS40/pOF105, have been use...

Authors: Ruriko Fukushima, Tetsuya Suzuki and Hiroyuki Kamiya

The importance of expert review to clarify ambiguous situations for (Q)SAR predictions under ICH M7

The use of in silico predictions for the assessment of bacterial mutagenicity under the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) M7 guideline is...

Authors: Robert S. Foster, Adrian Fowkes, Alex Cayley, Andrew Thresher, Anne-Laure D. Werner, Chris G. Barber, Grace Kocks, Rachael E. Tennant, Richard V. Williams, Steven Kane and Susanne A. Stalford

Effects of smoking cessation on biological monitoring markers in urine

Urinary nicotine and cotinine levels are often measured as biomarkers for tobacco smoke exposure. However, these biomarkers are not appropriate to evaluate the effects of quitting smoking for several days, bec...

Authors: Yuya Kawasaki, Yun-Shan Li, Yuko Ootsuyama, Kazuhiko Nagata, Hiroshi Yamato and Kazuaki Kawai

Mutagenic, Genotoxic and Immunomodulatory effects of Hydroxychloroquine and Chloroquine: a review to evaluate its potential to use as a prophylactic drug against COVID-19

Hydroxychloroquine (HCQ) and Chloroquine (CQ) are two anti-malarial drugs that are now being extensively used by front-line healthcare workers and other common people as a prophylactic drug against the Corona ...

Authors: Allan Giri, Ankita Das, Ajoy K. Sarkar and Ashok K. Giri

DNA hypomethylation drives changes in MAGE-A gene expression resulting in alteration of proliferative status of cells

Melanoma Antigen Genes (MAGEs) are a family of genes that have piqued the interest of scientists for their unique expression pattern. A subset of MAGEs (Type I) are expressed in spermatogonial cells and in no ...

Authors: Ashley Colemon, Taylor M. Harris and Saumya Ramanathan

An assessment of mutagenicity of chemical substances by (quantitative) structure–activity relationship

Currently, there are more than 100,000 industrial chemicals substances produced and present in our living environments. Some of them may have adverse effects on human health. Given the rapid expansion in the n...

Authors: Masamitsu Honma

In vitro toxicological assessment of gadolinium (III) chloride in V79–4 fibroblasts

Rare earth minerals of the lanthanide series are widely used in the field of medical and clinical application. Gadolinium (Gd), the most preferred rare earth mineral is frequently used as magnets, superconduct...

Authors: Ee Ling Siew, Ahmad Faizzudin Farris, Noramiwati Rashid, Kok Meng Chan and Nor Fadilah Rajab