Negative and positive control ranges in the bacterial reverse mutation test: JEMS/BMS collaborative study

A large-scale study was conducted by multiple laboratories affiliated with the Japanese Environmental Mutagen Society and the Bacterial Mutagenicity Study Group to investigate possible proficiency indicators f...

Authors: Masayuki Kato, Kei-ichi Sugiyama, Toshiro Fukushima, Yasuyoshi Miura, Takumi Awogi, Shigetomo Hikosaka, Kumiko Kawakami, Madoka Nakajima, Masato Nakamura, Hajime Sui, Kumiko Watanabe and Atsushi Hakura

Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD

Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as...

Authors: Kazuaki Kawai, Hiroshi Kasai, Yun-Shan Li, Yuya Kawasaki, Shintaro Watanabe, Masanori Ohta, Toru Honda and Hiroshi Yamato

Correlation between the results of in vitro and in vivo chromosomal damage tests in consideration of exposure levels of test chemicals

We examined the correlation between the results of in vitro and in vivo chromosomal damage tests by using in-house data of 18 pharmaceutical candidates that showed positive results in the in vitro chromosomal ...

Authors: Eiji Yamamura, Chinami Aruga, Shigeharu Muto, Nobuyuki Baba and Yoshifumi Uno

The 30th summer school of the Research Community for Mechanisms of Mutations

The 30th summer school of the Research Community for Mechanisms of Mutations was held on September 2nd-3rd, 2017 at the Kyoto Prefecture Seminar House. The Community celebrated the 30th anniversary of the scho...

Authors: Takashi Yagi, Masanobu Kawanishi and Kazuhiko Takahashi

The effect of different methods and image analyzers on the results of the in vivo comet assay

The in vivo comet assay is a widely used genotoxicity test that can detect DNA damage in a range of organs. It is included in the Organisation for Economic Co-operation and Development Guidelines for the Testi...

Authors: Takahiro Kyoya, Rika Iwamoto, Yuko Shimanura, Megumi Terada and Shuichi Masuda

Necessity for retrospective evaluation of past-positive chemicals in in vitro chromosomal aberration tests using recommended cytotoxicity indices

We have demonstrated that retrospective evaluation of existing data of in vitro chromosomal aberration test using the new cytotoxicity indices RICC (relative increase in cell count) or RPD (relative population...

Authors: Hiroshi Honda, Yurika Fujita, Toshio Kasamatsu, Anne Fuchs, Rolf Fautz and Osamu Morita

Increase of somatic cell mutations in oxidative damage-sensitive drosophila

Oxidative damage is an important genotoxic source for almost all organisms. To efficiently detect mutations induced by oxidative damage, we previously developed a urate-null Drosophila strain. Using this Droso...

Authors: Ryota Koike, Tomoyo Uchiyama, Sakae Arimoto-Kobayashi, Keinosuke Okamoto and Tomoe Negishi

Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end

Environmental factors stimulate alteration of DNA methylation level. Investigation of the genome-wide DNA methylation status is important for environmental health studies. We here designed a genomic DNA amplif...

Authors: Hisaka Kurita, Toshiki Aiba, Toshiyuki Saito and Seiichiroh Ohsako

Unique molecular mechanisms for maintenance and alteration of genetic information in the budding yeast Saccharomyces cerevisiae

The high-fidelity transmission of genetic information is crucial for the survival of organisms, the cells of which have the ability to protect DNA against endogenous and environmental agents, including reactiv...

Authors: Sayoko Ito-Harashima and Takashi Yagi

CiAPEX2 and CiP0, candidates of AP endonucleases in Ciona intestinalis, have 3′-5′ exonuclease activity and contribute to protection against oxidative stress

Apurinic/apyrimidinic (AP) sites are one of the most frequent DNA lesions. AP sites inhibit transcription and DNA replication, and induce cell death. AP endonucleases are key enzymes in AP site repair. Several...

Authors: Masafumi Funakoshi, Daisuke Nambara, Yuichiro Hayashi and Qiu-Mei Zhang-Akiyama

Protective effects of tea polyphenols and β-carotene against γ-radiation induced mutation and oxidative stress in Drosophila melanogaster

The commonly consumed antioxidants β-carotene and tea polyphenols were used to assess their protective effects against γ-radiation induced sex-linked recessive lethal (SLRL) mutation and oxidative stress in Droso...

Authors: Isha Nagpal and Suresh K. Abraham

Estimation of the effects of heavy Asian dust on respiratory function by definition type

The adverse effects of Asian dust (AD) on health have been demonstrated in earlier studies, but there is no standardized definition for heavy–AD. This study aimed to examine which definition of heavy–AD has th...

Authors: Jun Kurai, Masanari Watanabe, Hisashi Noma, Kyoko Iwata, Jumpei Taniguchi, Hiroyuki Sano, Yuji Tohda and Eiji Shimizu

Research on environmental mutagenesis from young scientists – the open symposium of the Japanese Environmental Mutagen Society (JEMS) in 2017

The open symposium of the Japanese Environmental Mutagen Society (JEMS) titled, “Research on Environmental Mutagenesis from Young Scientists,” was held at Kokusai Kenkyu Koryu Kaikan, the Foundation for Promot...

Authors: Kenichi Masumura and Shuichi Masuda

A perspective of Genes and Environment for the development of environmental mutagen research in Asia

Two years have passed since the Japanese Environmental Society (JEMS) made the official journal Genes and Environment (G&E) open access. Current subjects on environmental mutagen research to further advance this ...

Authors: Takashi Yagi

Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells

Blackcurrant (Ribs nigrum L.) is a classical fruit that has long been used to prepare juice, jam, liqueur, and sometimes medicines in Europe. Previously, we reported a genome defense effect by the antioxidative a...

Authors: Ayumi Yamamoto, Tokuhisa Hirouchi, Saori Kawamorita, Kana Nakashima, Atena Sugiyama and Yoji Kato

Generation, repair and replication of guanine oxidation products

Guanine is the most readily oxidized of the four DNA bases, and guanine oxidation products cause G:C-T:A and G:C-C:G transversions through DNA replication. 8-Oxo-7,8-dihydroguanine (8-oxoG) causes G:C-T:A tran...

Authors: Katsuhito Kino, Masayo Hirao-Suzuki, Masayuki Morikawa, Akane Sakaga and Hiroshi Miyazawa

Association between transported Asian dust and outdoor fungal concentration during winter in a rural area of western Japan

Recently, Asian dust (AD) has become a serious health problem and several studies have clearly proven that AD can aggravate asthma. However, it remains unclear as to which components of AD have a strong effect...

Authors: Kyoko Iwata, Masanari Watanabe, Jun Kurai, Naoto Burioka, Sachiko Nakamoto, Degejirihu Hantan and Eiji Shimizu

Further evidence for the association of CYP2D6*4 gene polymorphism with Parkinson’s disease: a case control study

Genetic and environmental risk factors play an important role for the susceptibility to sporadic Parkinson’s disease (PD). It was hypothesized that a splice variant of the CYP2D6 gene (CYP2D6*4 allele) is associa...

Authors: Anwarullah, Muhammad Aslam, Mazhar Badshah, Rashda Abbasi, Aneesa Sultan, Kafaitullah Khan, Nafees Ahmad and Jakob von Engelhardt

Erratum to: Genes and Environment, Vol. 39

Authors:

Giemsa-stained pseudo-micronuclei in rat skin treated with vitamin D₃ analog, pefcalcitol

Pefcalcitol, an analog of vitamin D₃ (VD₃), is an anti-psoriatic drug candidate that is designed to achieve much higher pharmacological effects, such as keratinocyte differentiation, than those of VD_3, with fewer...

Authors: Akira Takeiri, Kenji Tanaka, Asako Harada, Kaori Matsuzaki, Mariko Yano, Shigeki Motoyama, Chie Katoh and Masayuki Mishima

Effect of physicochemical character differences on the genotoxic potency of kaolin

Kaolin is white clay mineral with the chemical composition Al₂Si₂O₅(OH)₄, and many varieties of kaolins having different crystal structures are utilized in industrial, cosmetic and medical fields. To evaluate the...

Authors: Tatsuya Kato, Tatsushi Toyooka, Yuko Ibuki, Shuichi Masuda, Masatoshi Watanabe and Yukari Totsuka

Modulation of benzo[a]pyrene–DNA adduct formation by CYP1 inducer and inhibitor

Benzo[a]pyrene (BaP) is a well-studied pro-carcinogen that is metabolically activated by cytochrome P450 enzymes. Cytochrome P4501A1 (CYP1A1) has been considered to play a central role in the activation step, whi...

Authors: Kazuhiro Shiizaki, Masanobu Kawanishi and Takashi Yagi

New insight for metformin against bladder cancer

International Agency for Research on Cancer (IARC) estimated that bladder cancer is the ninth most common cancer in the world, with 430,000 new cases and 165,000 deaths in 2012. Bladder cancer represents the f...

Authors: Amr Ahmed EL-Arabey

Evaluation of in vivo mutagenesis for assessing the health risk of air pollutants

Various kind of chemical substances, including man-made chemical products and unintended products, are emitted to ambient air. Some of these substances have been shown to be mutagenic and therefore to act as a...

Authors: Yasunobu Aoki

Tumor-augmenting effects of gestational arsenic exposure on F1 and F2 in mice

The consequences of early-life exposure to chemicals in the environment are emerging concerns. Chronic exposure to naturally occurring inorganic arsenic has been known to cause various adverse health effects, ...

Authors: Keiko Nohara, Takehiro Suzuki, Kazuyuki Okamura, Junya Matsushita and Shota Takumi