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Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell

Abstract

Background

Heat shock proteins (Hsps) are overexpressed in several tumors and contribute to cell proliferation, metastasis, and anticancer drug resistance. Therefore, Hsp inhibitors have enhanced cytotoxicity as chemotherapeutic agents and may be effective with a reduced dosage for tumor therapy to avoid side effects.

Results

Four new azaphilones, maximazaphilones I–IV (14), and three known compounds (57) have been isolated from the airborne-derived fungus Penicillium maximae. Inhibitory effects of isolated compounds against induction of Hsp105 were evaluated by the luciferase assay system using Hsp105 promoter. In this assay, 24, 6, and 7 significantly inhibited hsp105 promoter activity without cytotoxicity. In addition, all isolated compounds except for 5 significantly induced the death of Adriamycin (ADR)-treated HeLa cells. Interestingly, 14, 6, and 7 didn’t show anti-proliferative and cell death-inducing activity without ADR.

Conclusion

This study revealed the chemical structures of maximazaphilones I–IV (14) and the potency of azaphilones may be useful for cancer treatment and reducing the dose of anticancer agents. In addition, one of the mechanisms of cell death-inducing activity for 24, 6, and 7 was suggested to be inhibitory effects of Hsp105 expression.

Introduction

Heat shock proteins (Hsps) play an important role in cellular homeostasis in response to stressors such as hypoxia, anoxia, high temperature, and several chemical agents that induce protein denaturation [1]. Hsps are overexpressed in several tumors and contribute to cell proliferation, metastasis, and anticancer drug resistance [2]. They are classified as Hsp27, Hsp40, Hsp60, Hsp70, Hsp90, and Hsp105 based on their molecular weights [3]. The anti-apoptotic function of Hsp70 and Hsp90 had been suggested to be one of the major mechanisms of chemotherapeutic agent resistance on cancer cells [4, 5]. Therefore, several Hsp70 and Hsp90 inhibitors are anticancer agents. Gambogic acid from Garcinia harburyi [6], derrubone from Derris robusta [7], and several azaphilones from Aspergillus deflectus [8] have been reported as Hsp90 inhibitors extracted from natural sources. A recent study revealed that decreased expression of Hsp105 enhances apoptosis induction against DNA-damaging agents, such as Adriamycin (ADR) [9]. However, the number of reported inhibitors of Hsp105 is limited [10, 11]. Considering these factors, our research group explored new inhibitors of Hsp expression, especially Hsp105.

To explore new Hsp expression inhibitors we selected azaphilones, secondary metabolites of fungi. Azaphilones have been isolated from Penicillium [12], Aspergillus [8], Chaetomium [13], Talaromyces [14], and Muyocopron [15] species. Among these fungi, more than 50 azaphilones were reported from the species of Penicillium section Sclerotiora [16,17,18,19] proving this species is the major source of azaphilones. Thus, we selected Penicillium maximae [20, 21] belonging to Penicillium section Sclerotiora from our library of airborne-derived fungi in Kyoto. In this study, we report chemical structures and inhibitory effects on Hsp expression of azaphilones produced by P. maximae JKYM-AK1.

Materials and methods

General experimental procedures

Specific rotations were obtained by using a JASCO P-2200 digital polarimeter (l = 5 cm). ECD spectroscopy was recorded using a JASCO J-1500 spectrometer. FAB-MS and HR-FAB-MS were recorded by using a JEOL JMS-SX 102 A mass spectrometer. 1 H NMR spectroscopy was recorded on JEOL ECS400 (400 MHz) and JNM-ECA 600 (600 MHz) spectrometers. 13 C NMR spectroscopy was recorded on a JNM-ECA 600 (150 MHz) spectrometer. 2D-NMR experiments were carried out on a JEOL JNM-ECA 600 (600 MHz) spectrometer.

Normal phase silica gel column chromatography was carried out using Silica gel 60 (Kanto Chemical Co., Inc. 63–210 mesh), and reversed phase silica gel column chromatography was carried out using C18-OPN (Nacalai Tesque Co., Inc. 140 μm). High-performance liquid chromatography (HPLC) was performed using a Shimadzu SPD-M20A UV–vis detector, Shimadzu LC-20AD pump, and Shimadzu SIL-20 A auto-injector. COSMOSIL 5C18-MS-II (Nacalai Tesque Co., Inc. 250 × 4.6 mm i.d., 250 × 10 mm i.d., and 250 × 20 mm i.d.) columns were used for analytical and preparative work.

Collection and identification of the JKYM-AK1 strain

Airborne particles (PM10, aerodynamic diameter ≤ 10 μm) were collected in the city of Kyoto (135.81°E, 34.99°N) using a high-volume air sampler (HV1000R, Shibata Scientific Technology, Soka, Japan) equipped with an impactor (Shibata Scientific Technology) in September 2021. The collected airborne particles were immediately suspended in distilled H2O. The suspension was inoculated with potato dextrose agar (PDA) and chloramphenicol (0.1 g/L) for incubating at 28 °C for 3 days. The JKYM-AK1 strain was isolated as colonies and stored in 10% glycerin at − 80 °C. Using internal transcribed spacer 1 (ITS1, 5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) as primers, the ITS1-5.8 S-ITS2 sequence region (511 base pairs, GenBank accession number ON150838) of JKYM-AK1 was obtained. BLAST analysis (NCBI database) showed that the sequence has a 99.60% identity of Penicillium maximae (Accession number: EU427298).

Fermentation and extraction

The seed culture of the fungus was prepared in a potato dextrose medium and incubated at 28 °C for 5 days at 200 rpm. The seed culture was added to 2 L × 20 Erlenmeyer flasks containing potato dextrose medium (1 L). The cultures were further incubated at 28 °C for 20 days followed by filtration. Mycelia was extracted three times with methanol by refluxing for 1 h. The solvent was evaporated to obtain a methanol extract. The methanol extract was combined with the supernatant and partitioned using EtOAc–water mixture.

Isolation of the compounds produced by the JKYM-AK1 strain

The EtOAc soluble fraction (8.31 g) was purified by normal phase silica gel column chromatography [n-hexane–CHCl3 (1:1 → 1:4→ 0:1, v/v) → CHCl3–MeOH (50:1 → 30:1 → 20:1 → 10:1 → 5:1 → 1:1, v/v)] and ten fractions were collected. Fraction 4 (2.3 g) was further separated using reversed phase silica gel column chromatography and eight fractions were collected. Fraction 4 − 3 (73.3 mg) was purified using HPLC {H2O–CH3CN (30:70, v/v)} to obtain 1 (3.2 mg), 2 (5.1 mg), 3 (8.4 mg), and 4 (7.1 mg). Fraction 4–4 (118.5 mg) was purified using HPLC {H2O–CH3CN (40:60, v/v)} to obtain 5 (48.9 mg). Fraction 5 (1.1 g) was separated using reversed phase silica gel column chromatography and ten fractions were obtained. Fraction 5 − 4 (22.2 mg) was purified using HPLC {H2O–CH3CN (60:40, v/v)} to obtain 7 (2.3 mg). Fraction 5–5 (23.3 mg) was purified using HPLC {H2O–CH3CN (65:35, v/v)} to obtain 6 (2.7 mg).

Maximazaphilone I (1)

Yellow amorphous solid; [α]25 D +44.2 (c 0.16, MeOH); 1 H NMR (CDCl3, 600 MHz) and 13 C NMR (CDCl3, 150 MHz), see Table 1; positive-ion FAB-MS m/z 385 [M + H]+; HR-FAB-MS m/z 385.2018 [M + H]+ (calcd for C23H29O5, 385.2015).

Maximazaphilone II (2)

Yellow amorphous solid; [α]25 D +100.3 (c 0.27, MeOH); 1 H NMR (CDCl3, 600 MHz) and 13 C NMR (CDCl3, 150 MHz), see Table 1; positive-ion FAB-MS m/z 319 [M + H]+; HR-FAB-MS m/z 319.1928 [M + H]+ (calcd for C19H27O4, 319.1909).

Maximazaphilone III (3)

Yellow amorphous solid; [α]25 D +54.4 (c 0.21, MeOH); 1 H NMR (CDCl3, 600 MHz) and 13 C NMR (CDCl3, 150 MHz), see Table 1; positive-ion FAB-MS m/z 361 [M + H]+; HR-FAB-MS m/z 361.2025 [M + H]+ (calcd for C21H29O5, 361.2015).

Maximazaphilone IV (4)

Yellow amorphous solid; [α]25 D +173.0 (c 0.15, MeOH); 1 H NMR (CDCl3, 600 MHz) and 13 C NMR (CDCl3, 150 MHz), see Table 1; positive-ion FAB-MS m/z 319 [M + H]+; HR-FAB-MS m/z 319.1919 [M + H]+ (calcd for C19H27O4, 319.1909).

Calculation of theoretical ECD spectra for maximazaphilone I, II, and IV (1, 2, and 4)

A preliminary molecular mechanics (MM) conformational search was carried out on 1, 2, and 4 in vacuum by using the Merck molecular force field (MMFF) as implemented in Spartan’16 program [22]. The stable energy conformers of each compound with Boltzmann distributions over 1% were further optimized at the B3LYP/def2-TZVP level of density functional theory (DFT). The normal mode analysis was done at the same level to confirm none of the conformers showed imaginary frequencies and to obtain the Gibbs free-energies [23]. The low free-energy conformers for each compound with Boltzmann distributions over 1% (Fig. S 4) were subjected to the ECD calculations using time-dependent density functional theory (TD-DFT) at the B3LYP/def2-TZVPP level. Both for the DFT and TD-DFT calculations were performed using an integral equation formalism polarizable continuum model (IEFPCM) in MeOH using Gaussian 16 [24]. The resultant rotatory strengths of the lowest 30 excited states for each conformer were converted into Gaussian-type curves with half-bands (0.30 eV) using SpecDis v1.71 [25]. The calculated ECD spectra of each of 1, 2, and 4 were composed after correction based on the Boltzmann distribution of conformers and their relative free-energy.

Cells

Human cervical carcinoma (HeLa) cells and mouse pGL105/C3H cells [26] were maintained in Dulbecco’s Modified Eagle Medium (DMEM) with low glucose (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 5% fetal bovine serum (Merck, Darmstadt, Germany) under a 5% CO2 atmosphere at 37 ℃.

Measurement of Luc activity

Stable hsp105 promoter-luciferase reporter cell lines (pGL105/C3H cells) were cultured in a flat-bottomed 96-well plate (Coster 3596; Corning, NY, USA) and incubated to reach 70 − 80% confluence. The cells were pretreated with test compounds for 30 min and exposed to heat shock at 41℃ for 3 h using a water bath. After washing the cells with PBS (-) (Wako Pure Chemical Industries) twice, 150 µL of 1X Glo Lysis Buffer (Promega, Madison, WI, USA) was added to each well and mixed by shaking for 20 min at room temperature. Luciferase activity and cell viability were measured on the 96-well white plate (136,101; Thermo Fisher Scientific, Waltham, MA, USA) by using a luminometer (GloMax® Discover System; Promega). Luciferase assay reagent (Promega) and CellTiter-Glo® 3D reagent (Promega) were used for the measurement of luminescence and cell viability, respectively.

Time-lapse imaging

Time-lapse imaging was performed using an Operetta high-content imaging system (PerkinElmer, Waltham, MA) as described previously [27]. The cells were cultured in a flat-bottomed 24-well plate (Coaster 3526; Corning) to reach 70 − 80% confluence. The cells were treated with test compounds or Adriamycin prior to the time-lapse cell imaging. Images were captured at 10 min intervals for 24 h under a 5% CO2 atmosphere at 37 ℃.

Evaluation of Hsps expression by western blot analysis

HeLa cells (1.0 × 105) were seeded in 35-mm dishes and cultured. After 24 h of incubation, the cells were treated with KRIBB11 or 6 for 30 min. Then, cells were exposed to 42 °C for 1 h for Hsps induction and were recovered at 37 °C for 5 h. Western blot analysis was performed as described previously [10]. Briefly, cells were lysed with SDS sample buffer and boiled at 100 °C for 5 min. Proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Pall Corporation, Port Washington, NY, USA). Blots were incubated with Blocking One reagent (Nacalai Tesque, Kyoto, Japan) and sequentially incubated with appropriate primary and secondary antibodies. Chemiluminescence was detected with an LAS-4000 mini-image analysis system (Fujifilm, Tokyo, Japan) using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA). Antibodies used in this study were as follows: mouse monoclonal anti-Hsp105 (1:1000–2000; clone B-7, Santa Cruz Biotechnology, Dallas, TX, USA), anti-Hsp90 (1:2000; clone AC88, Enzo Life Sciences, Farmingdale, NY, USA), anti-Hsp70 (1:2000; clone C92F3A-5, Enzo Life Sciences) and rabbit monoclonal anti-a-tubulin (1:2000; clone DM1A, Sigma-Aldrich, St. Louis, MO, USA) antibodies. HRP-conjugated donkey anti-mouse (1:4000; 712-035-151, Jackson Immuno Research Laboratories Inc., West Grove, PA, USA) and donkey anti-rabbit (1:4000; 712-035-152, Jackson Immuno Research Laboratories Inc.) IgG antibodies.

Statistical analysis

Statistical analysis was performed using GraphPad Prism 8.21 software. Statistical analysis was conducted using a one-way analysis of variance (ANOVA) followed by a Dunnett’s test or a Tukey–Kramer test to analyze the differences between the treatment groups. The significance level used for statistical analysis with two-tailed testing was 5%.

Results and discussion

Isolation of the compounds produced by P. maximae JKYM-AK1

The fungus, P. maximae JKYM-AK1 was cultured in a potato dextrose medium. The EtOAc soluble fraction was obtained from mycelia and supernatant after incubation of the culture at 28 °C for 20 days. The EtOAc soluble fraction was purified by normal and reversed phase silica gel column chromatography followed by HPLC to give four new compounds: maximazaphilones I–IV (1–4) together with three known compounds, isochromophilone IV (5) [28, 29], isochromophilone I (6) [30], and hypocrellone A (7) [31].

Chemical structure of maximazaphilone I (1)

Maximazaphilone I (1) was isolated as a yellow amorphous powder with positive optical rotation ([α]25 D +44.2 in MeOH). Its molecular formula (C23H28O5) was determined using HRMS and 13 C NMR spectroscopy. A molecular ion peak was observed using FAB-MS for 1 (m/z 385 [M]+). The 1 H and 13 C NMR (CDCl3) spectra recorded for 1 (Table 1) were similar to those of monascusone B [32] except for the presence of side chain. Namely, the 1 H and 13 C NMR spectra showed characteristic signals for three carbonyl groups [δ C 188.5 (C-9), 169.3 (C-21), and 200.3 (C-23)], two olefin groups [δ C 162.7 (C-3), 104.3 (C-4), 144.0 (C-5), and 114.6 (C-10)], a methylene bearing oxygen function group [δ Η 4.88 (d, J = 13.2, H-1) and 4.99 (d, J = 13.2, H-1)], a methylene group [δ Η 2.43 (overlapping with other signal, H-6b) and 2.88 (dd, J = 6.6, 19.2, H-6)], two methines [δ Η 3.26 (dd, J = 4.8, 12.6, H-7) and 3.59 (d, J = 12.6, H-22)], a quaternary carbon bearing oxygen function group [δ C 82.7 (C-8)] and two methyl groups [δ H 1.62 (s, H-20) and 2.46 (s, H-24)]. The positions of the side chain (C-11–C-19) was determined based on the DQF COSY and HMBC spectra shown in Fig. 1. Namely, the long-range correlations were observed between H-4/C-11 suggested the side chain was attached at C-3. The relative stereo structure except for C-15 and geometry of C-10–C-14 double bonds were determined by NOESY spectra. NOESY cross-peaks corresponding to H-6a/H-7, H-6a/H-20, H-7/H-20, and H-7/H-22 suggested that H-6a, H-7, H-22, and methyl group attached at C-8 were located at same side. NOESY cross-peaks corresponding to H-4/H-11, H-11/H-19, and H-12/H-14 established the geometry of C-11/12 and C-13/14 as E configurations. Finally, the absolute stereochemistry was determined by the calculated ECD spectra. The experimental ECD spectrum 250–400 nm for 1 was identical to that of calculated ECD spectra having 7R,8 S,15 S,22R and 7R,8 S,15R,22R absolute stereochemistry. On the other hand, the calculated ECD spectra of 7 S,8R,15 S,22 S were the opposite of the experimental data. Therefore, we concluded the absolute stereochemistry at the tricyclic ring is 7R,8 S,22R. In addition, the calculated ECD spectrum for the diastereomer of 1 having 15R absolute stereochemistry at side chain showed negative cotton effect at 436 nm and 1 didn’t show this trend, therefore, the absolute stereo structure of C-15 was determined as S (Fig. 2). Based on all this evidence, the chemical structures of maximazaphilone I (1) was determined as shown in Fig. 3.

Fig. 1
figure 1

Important 2D NMR correlations of new maximazaphilones (14)

Fig. 2
figure 2

Comparison of the experimental and calculated ECD spectra for 1, 2, and 4

Fig. 3
figure 3

The chemical structures of the isolated azaphilones (17) produced by P. maximae JKYM-AK1

Chemical structure of maximazaphilones II and III (2 and 3)

Maximazaphilones II and III (2 and 3) were isolated as a yellow amorphous powder with positive optical rotations (2, [α]25 D +100.3 in MeOH) (3, [α]25 D +54.4 in MeOH). The molecular formulas (2, C19H26O4) and (3, C21H28O5) were determined using HRMS and 13 C NMR spectroscopy. The 1 H and 13 C NMR (CDCl3) spectra recorded for 2 (Table 1) show signals very similar to that of asperpyranone [33], however, the absolute stereochemistry of C-7 has not been described. A comparative study of 2 with maximazaphilone III (3) indicated the presence of an additional acetyl group also proved by its molecular formula and 13 C NMR spectra [3, δ C (ppm) 170.6 (OCOCH3) and 21.1 (OCOCH3)]. The acetyl group in 3 is present at C-7 as established from the HMBC correlation between H-7/OCOCH3. NOESY cross peaks of 2 and 3 corresponding to [2, H-6a/H-7 and H-7/H-20] and [3, H-6a/H-20 and H-7/H-20] suggested that H-6a, H-7, and H-20 are located at the same side (Fig. 1). Therefore, the relative structures of 2 and 3 were determined as 7 S* and 8 S*. The absolute stereochemistry of C-15 was assumed to be S similar to 1 and other known azaphilones. The experimental ECD spectra of 2 and 3 were identical to that of the calculated ECD spectra having 7 S,8 S,15 S absolute stereochemistry. Conversely, the calculated ECD spectrum of ent-2 (7R,8R,15R) was opposite of the experimental data (Fig. 2). The absolute stereochemistry for 3 was deduced to be same as 2. Therefore, the absolute stereochemistry for both 2 and 3 was determined as 7 S,8 S,15 S. Based on these data, the determined chemical structures of maximazaphilone II and III (2 and 3) are shown in Fig. 3.

Table 1 13 C NMR (150 MHz) and 1 H NMR spectroscopic data (600 MHz) of 14 in CDCl3

Chemical structure of maximazaphilone IV (4)

Maximazaphilone IV (4) was isolated as a yellow amorphous powder with a positive optical rotation ([α]25 D +173.0 in MeOH). The molecular formula of 4 (C19H26O4) was determined using HRMS and 13 C NMR spectroscopy. The 1 H and 13 C NMR (CDCl3) spectra recorded for 4 (Table 1) show signals close to those of dechloroisochromophilone [34] and (8 S,9 S,10 S)-dechloroisochromophilone [35] except for the signals corresponding to C-7 [δ C 199.4], C-8 [δ C 76.4], C-9 [δ C 74.5], and C-10 [δ C 36.6]. These findings suggested that 4 should be the diastereomer of these two known compounds. NOESY cross-peaks of 4 corresponding to H-20/H-9, H-20/H-10, H-9/H-10 suggested that H-20, H-9, and H-10 were located at same side (Fig. 1). Therefore, the relative structure of 4 were determined as 8R*, 9R*, and 10 S*. The absolute stereochemistry of 4 was determined by the calculated ECD spectra and was found to be the same as 1–3 (Fig. 2). Based on these data, the chemical structure of maximazaphilone IV (4) was determined as shown in Fig. 3.

Evaluation of the inhibitory effects of isolated compounds against Hsp105 expression using luciferase assay system

Inhibitory effects of the isolated compounds (17) against the expression of Hsp105 were evaluated for heat shock promoter activity using a luciferase (luc) assay system using pGL105/C3H cells. pGL105/C3H cells were mouse C3H10T1/2 cells stably transfected with pGL105 reporter plasmid containing the Hsp105 promoter upstream of a luciferase gene. In this assay, the inhibitory effect of test samples was assessed by observing the decrease of Luc activity [26]. KRIBB11 was used as a positive control. KRIBB11 had been reported to be an inhibitor of the heat shock factor 1, the transcription factor of several Hsps including Hsp105 [3]. 27 significantly decreased Luc activity; however, 5 significantly inhibited cell proliferation (Fig. 4). These results suggested that 2–4, 6, and 7 should have inhibitory effects against Hsp105 expression on pGL105/C3H cells. We could not determine whether the inhibitory effect against Luc activity is caused by cytotoxicity in the case of 5.

Fig. 4
figure 4

The inhibitory effects of the isolated compounds (17) against heat shock promoter activity using a luciferase assay system with pGL105/C3H cells. KRIBB11 was used for positive control. The percentages of luciferase activities and cell viabilities were described as means ± SD (n = 3) from one of the three independent experiments. Statistical significance was analyzed using the Dunnett’s test (*P < 0.01, #P < 0.01 compared with each control group)

Evaluation of the cell death inducing activity on ADR-treated HeLa cells

Hsp105, a molecular chaperone, suppresses ADR-induced cell death via anti-apoptotic functions [9]. Our previous study suggested that the cell cycle arrest was induced via DNA damage, however, low concentrations of ADR (0.1 − 1.0 µg/ml) do not induce cell death [10]. Therefore, the compounds that inhibit the functions or expression of Hsp105 should increase the number of dead cells on low concentration ADR treatment cells. We evaluated the cell-death inducing activities of isolated compounds on HeLa cells for 24 h using time-lapse imaging analysis. In this study, we counted the number of mitotic entry cells and dead cells under treatment of test compounds (1 − 7) (30 µM) (Fig. 5A), ADR (1.0 and 2.0 µg/ml), and combination of test compounds (30 µM) with ADR (1.0 µg/ml) (Fig. 5B). Treatment of 14, 6, and 7 did not affect the number of dead cells and mitotic entry cells. On the other hand, 5 increased the number of dead cells and decreased the number of mitotic entry cells. The combination treatment of all isolated compounds (17) with ADR (1.0 µg/ml) significantly increased the dead cells compared to those of ADR-treated cells (1.0 µg/ml). Therefore, 14, 6, and 7 may suppress the anti-apoptotic functions of HeLa cells. Moreover, the number of dead cells by combination treatment of 6 with ADR (1.0 µg/ml) was significantly larger than that of ADR (2.0 µg/ml) treatment group. We also showed that HSF1 inhibitor KRIBB11 (positive control) increased ADR sensitivity (Fig. S4) and dose dependent cell death-inducing activity (Fig. S 5). These results suggest that 6 may be able to reduce the dose of ADR less than 50% to avoid the side effects.

Fig. 5
figure 5

Effects of the isolated compounds (1 − 7) on cell proliferation and death. The number of mitotic entry cells and dead cells were counted during time-lapse imaging. The percentages of mitotic entry cells or dead cells are reported as means ± SD of three different visual fields. Each field captured more than 100 cells. A HeLa cells were treated with 30 µM indicated compounds. Statistical significance was analyzed using the Tukey-Kramer test (*P < 0.01 compared with control group). B ADR (1.0 mg/ml) or combination of ADR (1.0 mg/ml) and isolated compounds (1 − 7) for 24 h [*P < 0.01 compared with ADR (1.0 mg/ml) group. # P < 0.01 compared with ADR (2.0 mg/ml) group

According to the results of luciferase assay and cell death-inducing activity, 6 may have the inhibitory effects against Hsp105 expression. Therefore, we evaluated the expression of Hsp105 and major Hsps, Hsp90 and Hsp70, in HeLa cells treated with 6 using western blotting analysis. Treatment with 100 µM 6 inhibited the expression of Hsp105 and Hsp90 under heat shock conditions (Fig. S6), however, 30µM 6 didn’t affect the expression of these Hsps (data not shown). Therefore, we concluded that the mechanisms of cell death inducing activity for 6 may be not only inhibition of the expression for Hsp105 and Hsp90 but also other effects that contributing to drug resistance.

Conclusion

We isolated new azaphilone, named maximazaphilones I–IV (1–4) together with known compounds (57) from the mycelia and the culture supernatant of P. maximae JKYM-AK1. Among the isolated compounds, 24, 6, and 7 were suggested to have inhibitory effects against Hsp105 expression on luciferase reporter assay using pGL105/C3H cells. The combination treatment of all isolated compounds with ADR (1.0 µg/ml) significantly increased the dead cells compared to those of ADR-treated cells (1.0 µg/ml). The previous report suggested that some azaphilones have the inhibitory effects against Hsp90 via interacting with the N-terminal domain [8], therefore, the mechanism of cell death inducing activity for 24, 6, and 7 against ADR-treated Hela cells may be not only inhibition of Hsp90 but also inhibition of the expression for Hsp105. Based on this evidence, we concluded that the azaphilone 24, 6, and 7 have a potency for cancer treatment to reduce the dose of anticancer agents via inhibition of Hsps expression including Hsp105.

Availability of data and materials

Not applicable.

References

  1. Lee HJ, Min HY, Yong YS, Ann J, Nguyen CT, La MT, Hyun SY, Le HT, Kim H, Kwon H, Nam G, Park HJ, Lee J, Lee HY. A novel C-terminal heat shock protein 90 inhibitor that overcomes STAT3-Wnt-b-catenin signaling-mediated drug resistance and adverse effects. Theranostics. 2022;12:105–25. https://doi.org/10.7150/thno.63788.

    Article  CAS  Google Scholar 

  2. Yun CW, Kim HJ, Lim JH, Lee SH. Heat shock proteins: agents of cancer development and therapeutic targets in anti-cancer therapy. Cells. 2019;9:60. https://doi.org/10.3390/cells9010060.

    Article  CAS  Google Scholar 

  3. Chatterjee S, Burns TF. Targeting heat shock proteins in cancer: a promising therapeutic approach. Int J Mol Sci. 2017;18:1978. https://doi.org/10.3390/ijms18091978.

    Article  CAS  Google Scholar 

  4. Sauvage F, Fattal E, Al-Sheer W, Denis S, Brotin E, Denoyelle C, Blanc-Fournier C, Toussaint B, Messaoudi S, Alami M, Barratt G, Vergnaud-Gauduchon J. Antitumor activity of nanoliposomes encapsulating the novobiocin analog 6BrCaQ in a triple-negative breast cancer model in mice. Cancer Lett. 2018;432:103–11. https://doi.org/10.1016/j.canlet.2018.06.001.

    Article  CAS  Google Scholar 

  5. Park SH, Kim WJ, Li H, Seo W, Park SH, Kim H, Shin SC, Zuiderweg ERP, Kim EE, Sim T, Kim NK, Shin I. Anti-leukemia activity of a Hsp70 inhibitor and its hybrid molecules. Sci Rep. 2017;7:3537. https://doi.org/10.1038/s41598-017-03814-6.

    Article  CAS  Google Scholar 

  6. Davenport J, Manjarrez JR, Peterson L, Krumm B, Blagg BSJ, Matts RL. Gambogic acid, a natural product inhibitor of Hsp90. J Nat Prod. 2011;74:1085–92. https://doi.org/10.1021/np200029q.

    Article  CAS  Google Scholar 

  7. Hadden MK, Galam L, Gestwicki JE, Matts RL, Blagg BSJ. Derrubone, an inhibitor of the Hsp90 protein folding machinery. J Nat Prod. 2007;70:2014–8. https://doi.org/10.1021/np070190s.

    Article  CAS  Google Scholar 

  8. Huo C, Lu X, Zheng Z, Li Y, Xu Y, Zheng H, Niu Y. Azaphilones with protein tyrosine phosphatase inhibitory activity isolated from the fungus aspergillus deflectus. Phytochemistry. 2020;170:112224. https://doi.org/10.1016/j.phytochem.2019.112224.

    Article  CAS  Google Scholar 

  9. Yamane T, Saito Y, Teshima H, Hagino M, Kakihana A, Sato S, Shimada M, Kato Y, Kuga T, Yamagishi N, Nakayama Y. Hsp105a suppresses adriamycin-induced cell death via nuclear localization signal‐dependent nuclear accumulation. J Cell Biochem. 2019;120:17951–62. https://doi.org/10.1002/jcb.29062.

    Article  CAS  Google Scholar 

  10. Matsumoto T, Kitagawa T, Imahori D, Yoshikawa H, Okayama M, Kobayashi M, Kojima N, Yamashita M, Watanabe T. Cell death-inducing activities via hsp inhibition of the sesquiterpenes isolated from Valeriana fauriei. J Nat Med. 2021;75:942–8. https://doi.org/10.1007/s11418-021-01543-9.

    Article  CAS  Google Scholar 

  11. Matsumoto T, Imahori D, Ohnishi E, Okayama M, Kitagawa T, Ohta T, Yoshida T, Kojima N, Yamashita M, Watanabe T. Chemical structures and induction of cell death via heat shock protein inhibition of the prenylated phloroglucinol derivatives isolated from Hypericum erectum. Fitoterapia. 2022;156:105097. https://doi.org/10.1016/j.fitote.2021.105097.

    Article  CAS  Google Scholar 

  12. Koyanagi Y, Hitora Y, Kawahara T, Peniphilones A. Azaphilone alkaloids from the Endophytic Fungus Penicillium maximae. Heterocycles. 2021;102:325–32. https://doi.org/10.3987/com-20-14373.

    Article  CAS  Google Scholar 

  13. Yang MY, Wang YX, Chang QH, Li LF, Liu YF, Cao F. Cytochalasans and azaphilones: suitable chemotaxonomic markers for the Chaetomium species. Appl Microbiol Biotechnol. 2021;105:8139–55. https://doi.org/10.1007/s00253-021-11630-2.

    Article  CAS  Google Scholar 

  14. de Oliveira F, Rocha ILD, Cláudia G, Alves Pinto D, Ventura SPM, Gonzaga Dos Santos A, José Crevelin E, et al. Identification of azaphilone derivatives of Monascus colorants from Talaromyces amestolkiae and their halochromic properties. Food Chem. 2022;372:131214. https://doi.org/10.1016/j.foodchem.2021.131214.

    Article  CAS  Google Scholar 

  15. Yuan C, Guo Y, Wang K, Wang Z, Li L, Zhu H, Li G. A novel azaphilone muyophilone A from the endophytic fungus Muyocopron laterale 0307-2. Front Chem. 2021;9. https://doi.org/10.3389/fchem.2021.734822.

  16. Zhao M, Ruan Q, Pan W, Tang Y, Zhao Z, Cui H. New polyketides and diterpenoid derivatives from the fungus penicillium sclerotiorum GZU-XW03-2 and their anti-inflammatory activity. Fitoterapia. 2020;143:104561. https://doi.org/10.1016/j.fitote.2020.104561.

    Article  CAS  Google Scholar 

  17. Tang JL, Zhou ZY, Yang T, Yao C, Wu LW, Li GY. Azaphilone alkaloids with anti-inflammatory activity from Fungus Penicillium sclerotiorum cib-411. J Agric Food Chem. 2019;67:2175–82. https://doi.org/10.1021/acs.jafc.8b05628.

    Article  CAS  Google Scholar 

  18. Jia Q, Du Y, Wang C, Wang Y, Zhu T, Zhu W. Azaphilones from the marine sponge-derived fungus Penicillium sclerotiorum OUCMDZ-3839. Mar Drugs. 2019;17:260. https://doi.org/10.3390/md17050260.

    Article  CAS  Google Scholar 

  19. Becker K, Pfütze S, Kuhnert E, Cox RJ, Stadler M, Surup F, Hybridorubrins. A–D: azaphilone heterodimers from stromata of Hypoxylon fragiforme and insights into the biosynthetic machinery for azaphilone diversification. Chemistry. 2021;27:1438–50. https://doi.org/10.1002/chem.202003215.

    Article  CAS  Google Scholar 

  20. Park MS, Chung D, Baek K, Lim YW. Three unrecorded species belonging to Penicillium Section Sclerotiora from marine environments in Korea. Mycobiology. 2019;47:165–72. https://doi.org/10.1080/12298093.2019.1601330.

    Article  Google Scholar 

  21. Wang XC, Chen K, Zeng ZQ, Zhuang WY. Phylogeny and morphological analyses of Penicillium section Sclerotiora (Fungi) lead to the discovery of five new species. Sci Rep. 2017;7:8233. https://doi.org/10.1038/s41598-017-08697-1.

    Article  CAS  Google Scholar 

  22. Irvine CA. Spartan’10, Wavefunction, USA, 2010.

  23. Pescitelli G, Bruhn T. Good computational practice in the assignment of Absolute Configurations by TDDFT calculations of ECD Spectra. Chirality. 2016;28:466–74. https://doi.org/10.1002/chir.22600.

    Article  CAS  Google Scholar 

  24. Frisch MJ, Trucks GW, Schlegel HB, Scuseria GE, Robb MA, Cheeseman JR. et al. Gaussian 09, Revision D.01, Gaussian Inc. Wallingford, CT 2009.

  25. Bruhn T, Schaumlöffel A, Hemberger Y, Bringmann G, SpecDis. Quantifying the comparison of calculated and experimental electronic circular dichroism spectra. Chirality. 2013;25:243–9. https://doi.org/10.1002/chir.22138.

    Article  CAS  Google Scholar 

  26. Ishihara K, Horiguchi K, Yamagishi N, Hatayama T. Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells. Eur J Biochem. 2003;270:3461–8. https://doi.org/10.1046/j.1432-1033.2003.03740.x.

    Article  CAS  Google Scholar 

  27. Μatsumoto T, Imahori D, Achiwa K, Saito Y, Ohta T, Yoshida T, Kojima N, Yamashita M, Nakayama Y, Watanabe T. Chemical structures and cytotoxic activities of the constituents isolated from Hibiscus tiliaceus. Fitoterapia. 2020;142:104524. https://doi.org/10.1016/j.fitote.2020.104524.

    Article  CAS  Google Scholar 

  28. Wang HC, Ke TY, Ko YC, Lin JJ, Chang JS, Cheng YB. Anti-inflammatory azaphilones from the edible alga-derived fungus penicillium sclerotiorum. Mar Drugs. 2021;19:529. https://doi.org/10.3390/md19100529.

    Article  CAS  Google Scholar 

  29. Arai N, Shiomi K, Tomoda H, Tabata N, Yang DJ, Masuma R, Kawakubo T, Ōmura S. Isochromophilones III–VI, inhibitors of acyl-CoA: cholesterol acyltransferase produced by Penicillium multicolor FO-3216. J Antibiot. 1995;48:696–702. https://doi.org/10.7164/antibiotics.48.696.

    Article  CAS  Google Scholar 

  30. Matsuzaki K, Tanaka H, Ōmura S, Isochromophilones I, II. Novel inhibitors against gpl20-CD4 binding produced by Penicillium multicolor FO-2338. J Antibio. 1995;48:708–13. https://doi.org/10.7164/antibiotics.48.708.

    Article  CAS  Google Scholar 

  31. Guo Q, Dong L, Zang X, Gu Z, He X, Yao L, Cao L, Qiu J, Guan X. A new azaphilone from the entomopathogenic fungus Hypocrella sp.Nat Prod Res.201529:2000–2006. https://doi.org/10.1080/14786419.2015.1023199.

  32. Jongrungruangchok S, Kittakoop P, Yongsmith B, Bavovada R, Tanasupawat S, Lartpornmatulee N, Thebtaranonth Y. Azaphilone pigments from a yellow mutant of the fungus Monascus kaoliang. Phytochemistry. 2004;65:2569–75. https://doi.org/10.1016/j.phytochem.2004.08.032.

    Article  CAS  Google Scholar 

  33. Chiang YM, Oakley CE, Ahuja M, Entwistle R, Schultz A, Chang SL, et al. An efficient system for heterologous expression of secondary metabolite genes in aspergillus nidulans. J Am Chem Soc. 2013;135:7720–31. https://doi.org/10.1021/ja401945a.

    Article  CAS  Google Scholar 

  34. Matsuzaki K, Tahara H, Inokoshi J, Tanaka H, Masuma R, Omura S. New brominated and halogen-less derivatives and structure-activity relationship of azaphilones inhibiting gp120-CD4 binding. J Antibiot (Tokyo). 1998;51:1004–11. https://doi.org/10.7164/antibiotics.51.1004.

    Article  CAS  Google Scholar 

  35. Pairet L, Wrigley SK, Chetland I, Reynolds EE, Hayes MA, Holloway J, Ainsworth AM, Katzer W, Cheng XM, Hupe DJ, Charlton P, Doherty AM. Azaphilones with endothelin receptor binding activity produced by Penicillium sclerotiorum: taxonomy, fermentation, isolation, structure elucidation and biological activity. J Antibiot (Tokyo). 1995;48:913–23. https://doi.org/10.7164/antibiotics.48.913.

    Article  CAS  Google Scholar 

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Acknowledgements

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Funding

This work was supported by JSPS KAKENHI Grant Numbers 20H03397 to TM.

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TM designed the study. TM, EO, TK, MO, YS, and HY contributed to the data collection. TO and TY contributed to the theoretical examination. TM drafted the manuscript, and YS, YN, and TW reviewed the manuscript. TM, YN, and TW supervised the study. All authors read and approved the final manuscript.

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Correspondence to Takahiro Matsumoto or Tetsushi Watanabe.

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Supplementary Information

Additional file 1: S1.

1H NMR spectra of maximazaphilones I–IV (14). S2. 13C NMR spectra of maximazaphilones I–IV (14). S3. TheDFT-optimized structures of conformers of maximazaphilones I, II, and IV (1, 2, and 4) with their equilibrium population. S4. Effects of the KRIBB11 (positive control) on cell proliferation and death. S5. Effects of the compounds and 7 at two concentrations (10 μM and 30 μM) on cell proliferation and death. S6. The expression of HSP on HeLa cell treated with 6.

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Matsumoto, T., Ohnishi, E., Kitagawa, T. et al. Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell. Genes and Environ 45, 5 (2023). https://doi.org/10.1186/s41021-023-00261-w

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Keywords

  • Penicillium maximae
  • Azaphilone
  • Maximazaphilone
  • Time-lapse imaging
  • Heat shock protein
  • JKYM-AK1
  • Adriamycin
  • Fungus