Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs41021-015-0014-8/MediaObjects/41021_2015_14_Fig3_HTML.gif)
Analysis of PCR products generated by RT-PCR from RNA transcripts of UV-irradiated DNA templates. a Scheme for detecting transcription inhibition using RNA transcripts from UV-irradiated (450Â J/m2) DNA templates. b Agarose gel (1Â %) demonstrating RNA transcripts from UV-irradiated DNA templates. M: size marker. Lane 1: RNA transcripts from mock DNA templates. Lane 2: RNA transcripts from UV-irradiated DNA templates. c Agarose gel (1Â %) demonstrating RT-PCR products (301Â bp) from UV-irradiated DNA templates. M: size marker. Odd lanes: RT-PCR products from mock DNA templates. Even lanes: RT-PCR products from UV-irradiated DNA templates. DNA was amplified for 9 (lanes 1 and 2), 12 (lanes 3 and 4), 15 (lanes 5 and 6), 18 (lanes 7 and 8), or 21 (lanes 9 and 10) PCR cycles. Lanes 11 and 12: no RT reaction