Table 2 Details of the 19 base substitutions called at the first screening in the control sample
From: Mutation assay using single-molecule real-time (SMRTTM) sequencing technology
Reference position | Reference | Forward read | Reverse read | Comment | Judgement | p-value** | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Most dominant allele | Coverage | Read count | p-value* | Most dominant allele | Coverage | Read count | p-value* | |||||
999271 | C | C | 33 | 23 | 3.9E-20 | T | 36 | 30 | 5.5E-31 | Mismatch | 0 | |
4778252 | T | T | 17 | 16 | 9.1E-19 | A | 19 | 19 | 4.4E-23 | Mismatch | 0 | |
3355477 | 0047 | C | 13 | 12 | 4.4E-14 | G | 11 | 10 | 1.0E-11 | Mismatch | 1.0E-11 | |
536849 | C | C | 9 | 9 | 2.5E-11 | T | 9 | 9 | 2.5E-11 | Mismatch | 5.0E-11 | |
1051080 | G | A | 10 | 9 | 1.6E-10 | G | 10 | 9 | 1.6E-10 | Mismatch | 3.1E-10 | |
3287776 | A | T | 11 | 8 | 8.2E-08 | A | 12 | 11 | 6.7E-13 | Mismatch | 8.2E-08 | |
3823422 | C | C | 7 | 4 | 5.7E-03 | G | 11 | 10 | 1.0E-11 | Mismatch | 5.7E-03 | |
316363 | G | edge of map | No | |||||||||
694963 | C | edge of map | No | |||||||||
918766 | G | edge of map | No | |||||||||
1922859 | C | edge of map | No | |||||||||
4423790 | C | edge of map | No | |||||||||
4144134 | C | No mutation | No | |||||||||
4515279 | C | No mutation | No | |||||||||
290717 | C | original allele | No | |||||||||
1760048 | A | original allele | No | |||||||||
1760052 | A | original allele | No | |||||||||
3741045 | T | original allele | No | |||||||||
4099877 | G | original allele | No | |||||||||