Fig. 3From: Progerin, the protein responsible for the Hutchinson-Gilford progeria syndrome, increases the unrepaired DNA damages following exposure to ionizing radiationInhibition of DSB repair foci formation by transient expression of GFP-progerin in NHDFs. a GFP-progerin plasmids were transiently expressed in early passage of MJ90 cells by lipofection, and formation of 53BP1 foci was monitored 1Â h after exposure to 1Â Gy (peak stage of repair foci formation). Typical examples of cell nuclei which showed strong (left) or weak (right) expression GFP-progerin are shown. b 53BP1 repair foci are predominantly formed in nuclei which expressed low levels of progerin (1Â Gy exposure and 1Â h incubation). Cells are rank-ordered by intensity of green fluorescenceBack to article page