Fig. 12
From: Genomic integration of lambda EG10 transgene in gpt delta transgenic rodents
Characterization of the phenotype of the gpt mutants rescued from gpt delta rats by spot testing. The cultured gpt mutant clones were spotted onto M9 with Cm plates (a) and M9 with Cm and 6TG plates (b) in dilution series. The experimental condition was described in Materials and Methods. Lane 1 and 2: Clones which have the gpt mutation at 299 T to A. Lane 3 and 4: Clones which have no gpt mutation. Lane 5 and 6: Positive control which has a gpt mutation (185 G to A in lane 5 and 416–418 GGG to GG in lane 6). Lane 7: Negative control which has no gpt mutation. The A:T to T:A transversion at position 299 did not alter 6TG sensitivity as a negative control. Positive control gpt mutants exhibited 6TG resistance