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Table 2 Summary of the mutation spectra of TK −/− mutantationsa

From: DNA polymerase kappa protects human cells against MMC-induced genotoxicity through error-free translesion DNA synthesis

  Solvent control MMC
  WT TK+/- KO TK+/- WT TK+/- KO TK +/-
Base substitution
 Transition     
  A:T → G:C 10 (36) 11 (26) 6 (6) 5 (4)
  G:C → A:T 1 (4) 2 (5) 5 (5) 4 (3)
 Transversion
  A:T → T:A 0 (0) 2 (5) 1 (1) 0 (0)
  A:T → C:G 0 (0) 2 (5) 4 (4) 1 (1)
  G:C → T:A 3 (11) 10 (23) 10 (11) 17 (13)
  G:C → C:G 0 (0) 1 (2) 8 (9) 9 (7)
 Tandem
  GG:CC → CT:GA 0 (0) 0 (0) 3 (3) 2 (2)
  GG:CC → TT:AA 0 (0) 0 (0) 8 (9) 6 (5)
  GG:CC → AT:TA 0 (0) 0 (0) 2 (2) 0 (0)
  CA:GT → TG:AC 1 (4) 0 (0) 0 (0) 0 (0)
  AG:TC → CT:GA 0 (0) 0 (0) 0 (0) 1 (1)
 Frameshift (+1) 0 (0) 0 (0) 1 (1) 1 (1)
 Frameshift (+2) 1 (4) 0 (0) 0 (0) 0 (0)
 Frameshift (−1) 0 (0) 0 (0) 0 (0) 2 (2)
 LOH 12 (43) 15 (35) 43 (46) 78 (62)
 Unidentified 0 (0) 0 (0) 2 (2) 0 (0)
 Total 28 (100) 43 (100) 93 (100) 126 (100)
  1. aData are presented as number of clones (ratio), with the results of two independent experiments are aggregated