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Table 3 Modulatory effects of TP and BC against γ-radiation induced oxidative stress on third instar larvae of D. melanogaster (ORK)

From: Protective effects of tea polyphenols and β-carotene against γ-radiation induced mutation and oxidative stress in Drosophila melanogaster

Parameters

aGSH

bGST

cCAT

dSOD

eLPO

Control

62.69 ± 3.66

37.39 ± 4.75

54.56 ± 4.30

3.56 ± 0.17

2.48 ± 0.30

TP 1%

76.56 ± 2.73###

46.28 ± 2.29#

64.68 ± 2.89#

3.95 ± 0.25

1.74 ± 0.21#

BC 0.25%

71.87 ± 1.58##

43.67 ± 3.72

53.01 ± 2.65

4.07 ± 0.10

2.07 ± 0.20

BC 0.5%

64.32 ± 3.24

41.12 ± 2.08

57.53 ± 4.50

3.74 ± 0.11

2.24 ± 0.22

BC 1%

64.26 ± 1.67

35.99 ± 4.79

51.91 ± 3.14

3.59 ± 0.21

2.08 ± 0.23

γ-radiation

49.41 ± 1.89###

23.63 ± 2.94###

31.08 ± 5.90###

1.45 ± 0.34###

5.70 ± 0.30###

γ-radiation + TP (1%)

61.97 ± 2.31***

{25.41%}

36.12 ± 2.55**

{35.63%}

53.44 ± 4.08***

{71.94%}

2.89 ± 0.22***, ##

{99.31%}

3.94 ± 0.17***, ###

{- 30.87%}

γ-radiation + BC (0.25%)

58.07 ± 1.80**

{17.52%}

34.28 ± 1.63**

{28.72%}

51.34 ± 2.56***

{65.18%}

2.93 ± 0.26***, ##

{102.0%}

2.48 ± 0.29***

{- 56.49%}

γ-radiation + BC (0.5%)

57.51 ± 2.18**, #

{16.39%}

36.28 ± 4.03**

{28.72%}

50.71 ± 4.28***

{63.15%}

2.51 ± 0.27***, ###

{73.10%}

2.60 ± 0.23***

{- 54.3%}

γ-radiation + BC (1%)

52.37 ± 2.67***, ###

{5.99%}

29.67 ± 3.08*

{11.41%}

38.52 ± 4.42*, ##

{23.93%}

2.20 ± 0.24***, ###

{51.72%}

2.84 ± 0.21***

{- 50.17%}

  1. Effect of TP and BC on (a) Reduced glutathione (GSH) levels expressed as nM of GSH formed mg−1 protein. (b) Glutathione S-transferase (GST) activity expressed as nM of CDNB conjugated min−1 mg−1 protein. (c) Catalase (CAT) activity expressed as μmoles of H2O2 decomposed min−1 mg−1 protein. (d) Superoxide dismutase (SOD) activity expressed as units min−1 mg−1 protein. (E) Lipid peroxidation (LPO) expressed as nmol of MDA formed h−1 mg−1 protein. Data represented as mean ± SD of three identical experiments. # p ≤ 0.05; ## p ≤ 0.01; ### p ≤ 0.001 compared with normal control group; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 compared with γ-radiation treated group
  2. The percentages in parentheses indicates the increase rates in the GSH, GST, CAT and SOD activity and decrease (-) in the LPO of test groups as compared to positive control group