Skip to main content
Fig. 3 | Genes and Environment

Fig. 3

From: Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end

Fig. 3

Electrophoretic patterns in the step-wise and sequencing of PCR product. a Lanes 1 and 8, just after the 1st Restriction enzyme digestion (HinP1 I and Nco I); lanes 2 and 9, after the 1st Adaptor ligation (Adaptor-1 and Adaptor-2); lanes 3 and 10, after the 1st PCR (M13 forward and M13 reverse primers); lanes 4 and 11, after the 2nd Restriction enzyme digestion (HinP1 I); lanes 5 and 12, after the 2nd Adaptor ligation (Adaptor-3); lanes 6 and 13, after the 2nd PCR as amplified methyl-CpG DNAs (T7 and M13 reverse); lanes 7 and 14, the products after PCR with gene-specific primers (T7 + Cyp1a1 and M13 + Cyp1a1). Around 100-bp position, two thick bands (100 bp and 110 bp) were detected after PCR with gene-specific primers (lanes 7 and 14). b Sequence result of 100-bp band. The sequence was matched with mouse Cyp1a1 promoter region indicted as 45-bp light blue characters in Fig. 2

Back to article page

Genes and Environment

ISSN: 1880-7062

Contact us